水稻中OsHox32的过表达对植株形态结构和叶片发育产生多效性影响。
Overexpression of OsHox32 Results in Pleiotropic Effects on Plant Type Architecture and Leaf Development in Rice.
作者信息
Li Ying-Ying, Shen Ao, Xiong Wei, Sun Qiong-Lin, Luo Qian, Song Ting, Li Zheng-Long, Luan Wei-Jiang
机构信息
College of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin, 300387, People's Republic of China.
出版信息
Rice (N Y). 2016 Dec;9(1):46. doi: 10.1186/s12284-016-0118-1. Epub 2016 Sep 13.
BACKGROUND
The Class III homeodomain Leu zipper (HD-Zip III) gene family plays important roles in plant growth and development. Here, we analyze the function of OsHox32, an HD-Zip III family member, and show that it exhibits pleiotropic effects on regulating plant type architecture and leaf development in rice.
RESULTS
Transgenic lines overexpressing OsHox32 (OsHox32-OV) produce narrow leaves that roll towards the adaxial side. Histological analysis revealed a decreased number of bulliform cells in OsHox32-OV lines. In addition, the angle between the leaf and culm was reduced, resulting in an erect plant phenotype. The height of the plants was reduced, resulting in a semi-dwarf phenotype. In addition, the chlorophyll level was reduced, resulting in a decrease in the photosynthetic rate, but water use efficiency was significantly improved, presumably due to the rolled leaf phenotype. OsHox32 exhibited constitutive expression in different organs, with higher mRNA levels in the stem, leaf sheath, shoot apical meristems and young roots, suggesting a role in plant-type and leaf development. Moreover, OsHox32 mRNA levels were higher in light and lower in the dark under both long-day and short-day conditions, indicating that OsHox32 may be associated with light regulation. Photosynthesis-associated and chlorophyll biosynthesis-associated genes were down-regulated to result in the reduction of photosynthetic capacity in OsHox32-OV lines. mRNA level of six rice YABBY genes is up-regulated or down-regulated by OsHox32, suggesting that OsHox32 may regulate the architecture of plant type and leaf development by controlling the expression of YABBY genes in rice. In addition, OsHox32 mRNA level was induced by the phytohormones, indicating that OsHox32 may be involved in phytohormones regulatory pathways.
CONCLUSIONS
OsHox32, an HD-Zip III family member, plays pleiotropic effects on plant type architecture and leaf development in rice.
背景
III类同源异型域亮氨酸拉链（HD-Zip III）基因家族在植物生长发育中发挥重要作用。在此，我们分析了HD-Zip III家族成员OsHox32的功能，并表明它在调节水稻株型结构和叶片发育方面表现出多效性。
结果
过表达OsHox32的转基因株系（OsHox32-OV）产生向近轴侧卷曲的窄叶。组织学分析显示OsHox32-OV株系中的泡状细胞数量减少。此外，叶片与茎秆之间的夹角减小，导致植株呈直立表型。植株高度降低，导致半矮化表型。此外，叶绿素水平降低，导致光合速率下降，但水分利用效率显著提高，可能是由于卷叶表型所致。OsHox32在不同器官中组成型表达，在茎、叶鞘、茎尖分生组织和幼根中的mRNA水平较高，表明其在株型和叶片发育中起作用。此外，在长日照和短日照条件下，OsHox32的mRNA水平在光照下较高，在黑暗中较低，表明OsHox32可能与光调节有关。光合作用相关和叶绿素生物合成相关基因下调，导致OsHox32-OV株系光合能力降低。六个水稻YABBY基因的mRNA水平被OsHox32上调或下调，表明OsHox32可能通过控制水稻中YABBY基因的表达来调节株型结构和叶片发育。此外，OsHox32的mRNA水平受植物激素诱导，表明OsHox32可能参与植物激素调节途径。
结论
HD-Zip III家族成员OsHox32在水稻株型结构和叶片发育中发挥多效性作用。
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