Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Department of Physics and Center for Biomedical Technology, Chung Yuan Christian University, Taoyuan, Taiwan.
Sci Rep. 2016 Sep 14;6:33414. doi: 10.1038/srep33414.
The application of optical absorption spectra in prognostic prediction has hardly been investigated. We developed and evaluated a novel two dimensional absorption spectrum measurement system (TDAS) for use in early diagnosis, evaluating response to chemoradiation, and making prognostic prediction. The absorption spectra of 120 sets of normal and tumor tissues from esophageal cancer patients were analyzed with TDAS ex-vivo. We demonstrated the cancerous tissue, the tissue from patients with a poor concurrent chemoradiotherapy (CCRT) response, and the tissue from patients with an early disease progression each had a readily identifiable common spectral signature. Principal component analysis (PCA) classified tissue spectra into distinct groups, demonstrating the feasibility of using absorption spectra in differentiating normal and tumor tissues, and in predicting CCRT response, poor survival and tumor recurrence (efficiencies of 75%, 100% and 85.7% respectively). Multivariate analysis revealed that patients identified as having poor-response, poor-survival and recurrence spectral signatures were correlated with increased risk of poor response to CCRT (P = 0.012), increased risk of death (P = 0.111) and increased risk of recurrence (P = 0.030) respectively. Our findings suggest that optical absorption microscopy has great potential to be a useful tool for pre-operative diagnosis and prognostic prediction of esophageal cancer.
光学吸收光谱在预后预测中的应用几乎未被研究过。我们开发并评估了一种新型的二维吸收光谱测量系统（TDAS），用于早期诊断、评估放化疗反应和进行预后预测。我们用 TDAS 对 120 组食管癌患者的正常和肿瘤组织的吸收光谱进行了分析。我们发现，癌症组织、对同期放化疗反应差的患者组织和疾病早期进展的患者组织都具有可识别的共同光谱特征。主成分分析（PCA）将组织光谱分为不同的组，证明了吸收光谱在区分正常和肿瘤组织以及预测放化疗反应、不良生存和肿瘤复发方面的可行性（效率分别为 75%、100%和 85.7%）。多变量分析表明，被鉴定为具有不良反应、不良生存和复发光谱特征的患者与对放化疗反应不良的风险增加（P=0.012）、死亡风险增加（P=0.111）和复发风险增加（P=0.030）相关。我们的研究结果表明，光学吸收显微镜具有成为食管癌术前诊断和预后预测的有用工具的巨大潜力。
