在p62/SQSTM1基因缺陷的小鼠胚胎成纤维细胞中，日本脑炎病毒复制受损。
Impaired Japanese encephalitis virus replication in p62/SQSTM1 deficient mouse embryonic fibroblasts.
作者信息
Tasaki Takafumi, Nukuzuma Souichi, Takegami Tsutomu
机构信息
Division of Protein Regulation Research, Department of Life Science, Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa 920-0293, Japan.
Department of Infectious Diseases, Kobe Institute of Health, 4-6-5, Minatojima-Nakamachi, Chuo-ku, Kobe 650-0046, Japan.
出版信息
Microbiol Immunol. 2016 Oct;60(10):708-711. doi: 10.1111/1348-0421.12440.
The role of the autophagy adaptor protein p62/SQSTM1 in Japanese encephalitis virus (JEV) replication in mouse embryonic fibroblasts (MEFs) was investigated. Amounts of JEV RNA and E protein were significantly smaller in p62-deficient cells than wild-type cells at 24 hr post-infection (p.i.). JEV RNA quantitation and viral plaque assays showed significant reductions in viral titers in p62-deficient cell culture fluid. Our results indicate that JEV replication is impaired in p62-deficient MEFs, suggesting that p62 positively regulates JEV replication in host cells.
研究了自噬衔接蛋白p62/SQSTM1在日本脑炎病毒（JEV）于小鼠胚胎成纤维细胞（MEF）中复制过程中的作用。在感染后24小时（p.i.），p62缺陷细胞中的JEV RNA和E蛋白量显著低于野生型细胞。JEV RNA定量和病毒蚀斑试验表明，p62缺陷细胞培养液中的病毒滴度显著降低。我们的结果表明，p62缺陷的MEF中JEV复制受损，提示p62在宿主细胞中正向调节JEV复制。
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