肥胖状态下孕期驱动的心血管系统母体miR-29可塑性
Pregnancy-driven cardiovascular maternal miR-29 plasticity in obesity.
作者信息
Schlabritz-Loutsevitch N, Apostolakis-Kyrus K, Krutilina R, Hubbard G, Kocak M, Janjetovic Z, Sathanandam S, Slominski A T, Mari G, Dick E
机构信息
Department of Obstetrics and Gynecology, Texas Tech University Health Sciences Center at the Permian Basin, Odessa, TX, USA.
Department of Obstetrics and Gynecology, University of Tennessee Health Science Center, Memphis, TN, USA.
出版信息
J Med Primatol. 2016 Dec;45(6):297-303. doi: 10.1111/jmp.12236. Epub 2016 Sep 15.
BACKGROUND
Obesity in pregnancy (MO) is a risk factor for maternal and/or fetal cardiovascular system disorders. This study evaluated maternal CVS expression of microRNA-29 family and its target molecules in MO to test the hypotheses: CVS miR-29 concentrations are increased in pregnancy and decreased in MO.
METHODS
Non-pregnant (n=4), pregnant obese (POb, n=4), and pregnant non-obese (PnOb, n=4) baboons (Papio spp.) were studied. Maternal left ventricle (LV), left atrium (LA), and aortic arch (AA) were collected at the end of gestation. Expression of MiR-29 and elastin (ELN) mRNA were quantified.
RESULTS
LA miR-29 (a, c) expression was highest in PnOb. In the LV, miR-29b expression trended lower (P=.059) for PnOb animals. ELN mRNA expression correlated positively with miR-29b expression in AA (r=.76, P=.03).
CONCLUSION
Maternal obesity diminishes miR-29 adaptation to pregnancy. Pharmacologic, tissue-specific targeting of miRNA-29 may represent a strategy for prevention and treatment of MO complications.
背景
孕期肥胖（MO）是孕产妇和/或胎儿心血管系统疾病的一个危险因素。本研究评估了孕期肥胖中微小RNA-29家族及其靶分子在母体心血管系统（CVS）中的表达，以验证以下假设：CVS中miR-29浓度在孕期升高，在MO中降低。
方法
研究了非孕（n = 4）、孕肥胖（POb，n = 4）和孕非肥胖（PnOb，n = 4）的狒狒（狒狒属）。在妊娠末期收集母体左心室（LV）、左心房（LA）和主动脉弓（AA）。对MiR-29和弹性蛋白（ELN）mRNA的表达进行定量。
结果
LA中miR-29（a，c）表达在PnOb中最高。在LV中，PnOb动物的miR-29b表达呈下降趋势（P = 0.059）。AA中ELN mRNA表达与miR-29b表达呈正相关（r = 0.76，P = 0.03）。
结论
母体肥胖会削弱miR-29对妊娠的适应性。对miRNA-29进行药物性、组织特异性靶向治疗可能是预防和治疗MO并发症的一种策略。
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