DO Carmo Natalia Gurgel, Sakamoto Luis Henrique Toshihiro, Pogue Robert, DO Couto Mascarenhas Cintia, Passos Simone Karst, Felipe Maria Sueli Soares, DE Andrade Rosângela Vieira
Program of Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, DF, Brazil Program of Molecular Pathology, University of Brasília, Brasília, DF, Brazil.
Domingos A. Boldrini Center for Hematological Investigation, Campinas, SP, Brazil.
Anticancer Res. 2016 Sep;36(9):4545-51. doi: 10.21873/anticanres.11002.
BACKGROUND/AIM: Nodular and superficial are the most common subtypes of basal cell carcinoma (BCC). Signaling pathways such as Hedgehog (HH) and Wingless (WNT) signaling are associated with BCC phenotypic variation. The aim of the study was to evaluate of the expression profiles of 84 genes related to the WNT and HH signaling pathways in patients with nodular and superficial BCC.
A total of 58 BCCs and 13 samples of normal skin were evaluated by quantitative real-time polymerase chain reaction (qPCR) to detect the gene-expression profile.
qPCR array showed segregation in BCC subtypes compared to healthy skin. PRKX, WNT3 and WNT16 were significantly (p<0.05) altered: PRKX was up-regulated, and WNT3 and WNT16 were down-regulated in nodular BCC.
PRKX, WNT3 and WNT16 genes, belonging to the WNT signaling pathway, are involved in the tumorigenic process of nodular BCC.
背景/目的：结节型和浅表型是基底细胞癌（BCC）最常见的亚型。刺猬信号通路（HH）和无翅信号通路（WNT）等信号通路与BCC的表型变异有关。本研究的目的是评估结节型和浅表型BCC患者中84个与WNT和HH信号通路相关基因的表达谱。
通过定量实时聚合酶链反应（qPCR）对58例BCC和13例正常皮肤样本进行评估，以检测基因表达谱。
与健康皮肤相比，qPCR阵列显示BCC亚型存在分离。PRKX、WNT3和WNT16有显著（p<0.05）变化：在结节型BCC中，PRKX上调，WNT3和WNT16下调。
属于WNT信号通路的PRKX、WNT3和WNT16基因参与了结节型BCC的致瘤过程。
