分泌途径激酶的纯化与检测方法

Methods to Purify and Assay Secretory Pathway Kinases.

作者信息

Tagliabracci Vincent S, Wen Jianzhong, Xiao Junyu

机构信息

Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX, 75390-9148, USA.

Discovery Bioanalytics, Merck and Co, Rahway, NJ, USA.

出版信息

Methods Mol Biol. 2016;1496:197-215. doi: 10.1007/978-1-4939-6463-5_16.

DOI:10.1007/978-1-4939-6463-5_16

PMID:27632012

Abstract

Members of the four-jointed and VLK families of secretory pathway kinases appear to be responsible for the phosphorylation of secreted proteins and proteoglycans. These enzymes have been implicated in many biological processes and mutations in several of these kinases cause human diseases. Here, we describe methods to purify and assay two members of the four-jointed family of secretory kinases: the Fam20C protein kinase and the Fam20B proteoglycan kinase.

摘要

分泌途径激酶的四联体家族和VLK家族成员似乎负责分泌蛋白和蛋白聚糖的磷酸化。这些酶参与了许多生物学过程，其中几种激酶的突变会导致人类疾病。在这里，我们描述了纯化和检测分泌激酶四联体家族的两个成员的方法：Fam20C蛋白激酶和Fam20B蛋白聚糖激酶。