Dang Baoqi, Shen Haitao, Li Haiying, Zhu Min, Guo Chunhua, He Weichun
Department of Neurosurgery, Zhangjiagang Hospital of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Suzhou, Jiangsu 215600, P.R. China.
Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
Mol Med Rep. 2016 Nov;14(5):4279-4284. doi: 10.3892/mmr.2016.5736. Epub 2016 Sep 13.
Our previous study determined that prominent cerebral vasospasm (CVS) may occur in an in vivo model of subarachnoid hemorrhage (SAH) in rats. Matrix metalloproteinase 9 (MMP‑9) expression levels in basilar arteries were upregulated in a similar manner to the development of CVS following SAH. To identify the changes that occur in the contractility of cerebrovascular smooth muscle cells and the expression levels of MMP‑9 in an in vitro model of SAH, rat cerebrovascular smooth muscle cells were isolated, cultured, and then stimulated with hemolysate. Additionally, 2-[(4-phenoxyphenylsulfonyl)methyl]thiirane (SB-3CT), a selective MMP-9 inhibitor, was used to determine the effect of MMP‑9 on the contractility of cerebrovascular smooth muscle cells. Cerebrovascular smooth muscle cells were successfully isolated and cultured in vitro, and hemolysate stimulation enhanced their contractility and increased MMP‑9 expression levels. The present study also revealed that pretreatment with SB‑3CT decreased MMP‑9 expression levels in cerebrovascular smooth muscle cells, and reduced their contractility upon hemolysate treatment. Therefore, the current study confirmed that MMP‑9 is important for the enhancement of the contractility of cerebrovascular smooth muscle cells in an in vitro rat model of SAH.
我们之前的研究确定，在大鼠蛛网膜下腔出血（SAH）的体内模型中可能会出现明显的脑血管痉挛（CVS）。SAH后，基底动脉中基质金属蛋白酶9（MMP-9）的表达水平与CVS的发展以相似的方式上调。为了确定在SAH的体外模型中脑血管平滑肌细胞收缩性以及MMP-9表达水平发生的变化，分离、培养大鼠脑血管平滑肌细胞，然后用溶血产物进行刺激。此外，使用选择性MMP-9抑制剂2-[(4-苯氧基苯基磺酰基)甲基]噻烷（SB-3CT）来确定MMP-9对脑血管平滑肌细胞收缩性的影响。脑血管平滑肌细胞成功地在体外分离和培养，溶血产物刺激增强了它们的收缩性并增加了MMP-9表达水平。本研究还表明，用SB-3CT预处理可降低脑血管平滑肌细胞中MMP-9表达水平，并降低溶血产物处理后它们的收缩性。因此，当前研究证实，在大鼠SAH体外模型中，MMP-9对于增强脑血管平滑肌细胞的收缩性很重要。
