松萝酸对耐甲氧西林金黄色葡萄球菌的抗菌及抗生物膜机制评估
Evaluation of antibacterial and antibiofilm mechanisms by usnic acid against methicillin-resistant Staphylococcus aureus.
作者信息
Pompilio Arianna, Riviello Antonella, Crocetta Valentina, Di Giuseppe Fabrizio, Pomponio Stefano, Sulpizio Marilisa, Di Ilio Carmine, Angelucci Stefania, Barone Luana, Di Giulio Andrea, Di Bonaventura Giovanni
机构信息
Department of Medical, Oral & Biotechnological Sciences, 'G d'Annunzio' University of Chieti-Pescara, Via Vestini 31, Chieti, Italy.
Aging Research Center and Translational Medicine, 'G d'Annunzio' University of Chieti-Pescara, Via L Polacchi 13, Chieti, Italy.
出版信息
Future Microbiol. 2016 Oct;11:1315-1338. doi: 10.2217/fmb-2016-0049. Epub 2016 Sep 16.
AIM
To evaluate the antibacterial and antibiofilm mechanisms of usnic acid (USN) against methicillin-resistant Staphylococcus aureus from cystic fibrosis patients.
MATERIALS & METHODS: The effects exerted by USN at subinhibitory concentrations on S. aureus Sa3 strain was evaluated by proteomic, real-time PCR and electron microscopy analyses.
RESULTS & CONCLUSION: Proteomic analysis showed that USN caused damage in peptidoglycan synthesis, as confirmed by microscopy. Real-time PCR analysis showed that antibiofilm activity of USN is mainly due to impaired adhesion to the host matrix binding proteins, and decreasing lipase and thermonuclease expression. Our data show that USN exerts anti-staphylococcal effects through multitarget inhibitory effects, thus confirming the rationale for considering it 'lead compound' for the treatment of cystic fibrosis infections.
目的
评估松萝酸（USN）对囊性纤维化患者耐甲氧西林金黄色葡萄球菌的抗菌及抗生物膜机制。
材料与方法
通过蛋白质组学、实时荧光定量聚合酶链反应（PCR）及电子显微镜分析，评估亚抑菌浓度的松萝酸对金黄色葡萄球菌Sa3菌株的作用。
结果与结论
蛋白质组学分析表明松萝酸导致肽聚糖合成受损，显微镜检查证实了这一点。实时荧光定量PCR分析表明，松萝酸的抗生物膜活性主要是由于其对宿主基质结合蛋白的黏附受损，以及脂肪酶和热核酸酶表达降低。我们的数据表明，松萝酸通过多靶点抑制作用发挥抗葡萄球菌作用，从而证实了将其视为治疗囊性纤维化感染“先导化合物”的合理性。
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