Thomas L H, Gourlay R N, Wyld S G, Parsons K R, Chanter N
Agricultural and Food Research Council, Compton Laboratory, Newbury, Berkshire, England.
Vet Pathol. 1989 May;26(3):253-9. doi: 10.1177/030098588902600310.
Five calves were inoculated intravenously with 10(8) colony forming units (cfu) of Pasteurella haemolytica A1; the mean score for pneumonic consolidation 3 days post-inoculation was 28%, and the mean clinical score was 7.8. Five calves inoculated intratracheally with 10(9) cfu of the same strain of P. haemolytica had comparable scores (34% and 8.8). Histological lesions of fibrinous pneumonia were similar in all calves. P. haemolytica was recovered from all but one of the affected lungs. From one calf killed in extremis 3 hours after intravenous inoculation, numbers of bacteria recovered from lung were 1,000-fold greater than from liver and spleen. A similar difference in bacterial numbers was also obtained from a gnotobiotic calf killed in extremis, 12 hours after intravenous inoculation of 10(8) cfu P. haemolytica. Evidence from these experiments supports the hypothesis that the blood-borne route is important in the pathogenesis of bovine pneumonic pasteurellosis.
给5头小牛静脉注射10⁸ 个溶血巴斯德氏菌A1菌落形成单位（cfu）；接种后3天，肺部实变的平均评分为28%，临床平均评分为7.8。给另外5头小牛气管内接种10⁹ cfu同一菌株的溶血巴斯德氏菌，其评分相近（34%和8.8）。所有小牛的纤维素性肺炎组织学病变相似。除一头患病小牛外，在所有患病小牛的肺中均分离到溶血巴斯德氏菌。在静脉接种后3小时濒死处死的一头小牛中，从肺中分离到的细菌数量比从肝脏和脾脏中分离到的多1000倍。在无菌小牛静脉接种10⁸ cfu溶血巴斯德氏菌12小时后濒死处死，也得到了类似的细菌数量差异。这些实验证据支持血行传播途径在牛肺巴氏杆菌病发病机制中起重要作用这一假说。
