牛无浆体病中白细胞和红细胞氧化防御系统的酶

Enzymes of oxidant defence system of leucocytes and erythrocytes in bovine anaplasmosis.

作者信息

More T, Reddy G R, Sharma S P, Singh L N

机构信息

Division of Biochemistry and Food Science, Indian Veterinary Research Institute, U.P., India.

出版信息

Vet Parasitol. 1989 Jun;31(3-4):333-7. doi: 10.1016/0304-4017(89)90082-4.

DOI:10.1016/0304-4017(89)90082-4

PMID:2763451

Abstract

The glutathione oxidant defence system in leucocytes and erythrocytes of six Anaplasma marginale-infected calves was examined by assaying glutathione peroxidase (GSH-px), glutathione reductase (GSSG-R), reduced glutathione (GSH) and superoxide dismutase (SOD). In addition, GSH-S-transferase and arginase levels were measured in leucocytes. There was a significant decline in the activities of leucocyte GSH-px, GSSG-R and SOD in the post-patent period; whereas arginase activity rose significantly following the patent period of anaplasmosis. The activity of erythrocyte SOD declined in the post-patent stage. The infection also caused a decline in red cell GSH (P less than 0.05). The results suggest that the glutathione oxidant defence system of peripheral blood cells is significantly influenced by the Anaplasma infection.

摘要

通过检测谷胱甘肽过氧化物酶（GSH-px）、谷胱甘肽还原酶（GSSG-R）、还原型谷胱甘肽（GSH）和超氧化物歧化酶（SOD），对6头感染边缘无浆体的犊牛白细胞和红细胞中的谷胱甘肽氧化防御系统进行了研究。此外，还检测了白细胞中谷胱甘肽-S-转移酶和精氨酸酶的水平。在感染后期，白细胞GSH-px、GSSG-R和SOD的活性显著下降；而在无浆体病的感染后期，精氨酸酶活性显著升高。感染后期红细胞SOD活性下降。感染还导致红细胞GSH水平下降（P<0.05）。结果表明，边缘无浆体感染显著影响外周血细胞的谷胱甘肽氧化防御系统。