实验感染边缘无形体的水牛（Bubalus bubalis）的氧化防御系统。

The oxidant defence system in water-buffaloes (Bubalus bubalis) experimentally infected with Anaplasma marginale.

作者信息

Reddy G R, More T, Sharma S P, Singh L N

机构信息

Division of Biochemistry and Food Science, Indian Veterinary Research Institute, Izatnagar.

出版信息

Vet Parasitol. 1988 Mar;27(3-4):245-9. doi: 10.1016/0304-4017(88)90039-8.

DOI:10.1016/0304-4017(88)90039-8

PMID:3369075

Abstract

The glutathione (GSH) -oxidant defence system protects the erythrocytes and leucocytes from oxidative damage. Leucocyte -superoxide dismutase (SOD), GSH-peroxidase (GSH-px), GSH-reductase (GR), GSH-S-transferase (GSH-S-t) and arginase were examined in samples from buffaloes infected with Anaplasma marginale. All the enzymes, except arginase, were also studied in the red cell haemolysates from these animals. GSH-S-t, GSH- and glutathione-reductase (GR) levels in leucocytes decreased in infected animals suggesting a decline in the efficiency of the GSH-oxidant defence system. SOD levels increased but there was no change in leucocyte-arginase activity due to infection. Infection caused no significant changes in red cell SOD, GSH-px, GR and GSH. However, GSH-S-t significantly decreased (P less than 0.05).

摘要

谷胱甘肽（GSH）-抗氧化防御系统可保护红细胞和白细胞免受氧化损伤。对感染边缘无形体的水牛样本中的白细胞超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-px）、谷胱甘肽还原酶（GR）、谷胱甘肽-S-转移酶（GSH-S-t）和精氨酸酶进行了检测。除精氨酸酶外，还对这些动物的红细胞溶血产物中的所有酶进行了研究。感染动物的白细胞中GSH-S-t、GSH和谷胱甘肽还原酶（GR）水平降低，表明GSH-抗氧化防御系统的效率下降。SOD水平升高，但感染并未导致白细胞精氨酸酶活性发生变化。感染对红细胞SOD、GSH-px、GR和GSH无显著影响。然而，GSH-S-t显著降低（P小于0.05）。