二甲基亚砜在液氮中用于胎儿三毛滴虫冷冻保存的应用。
Use of DMSO for the cryopreservation of Tritrichomonas foetus in liquid nitrogen.
作者信息
Campero C M
机构信息
Departamento de Producción Animal EEA INTA Balcarce C.C., Buenos Aires, Argentina.
出版信息
Vet Parasitol. 1989 Jun;31(3-4):339-43. doi: 10.1016/0304-4017(89)90083-6.
PMID:2763452
Abstract
Four Tritrichomonas foetus strains were successfully preserved in liquid nitrogen at -196 degrees C in the presence of 10% dimethylsulfoxide (DMSO) for 7 months (Strain 1) and 6 months (Strain 2, 3 and 4). The percentage of viable organisms after thawing ranged between 65 and 85%, depending upon the strain. The method used is described in detail.
摘要
四株胎儿三毛滴虫在含有10%二甲基亚砜（DMSO）的条件下于-196℃液氮中成功保存了7个月（菌株1）以及6个月（菌株2、3和4）。解冻后活生物体的百分比在65%至85%之间，具体取决于菌株。文中详细描述了所使用的方法。
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