Valkenburg Kenneth C, Amend Sarah R, Verdone James E, van der Toom Emma E, Hernandez James R, Gorin Michael A, Pienta Kenneth J
The James Buchanan Brady Urological Institute and Department of Urology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Oncotarget. 2016 Oct 25;7(43):69794-69803. doi: 10.18632/oncotarget.12000.
Bone metastasis is a lethal and incurable disease. It is the result of the dissemination of cancer cells to the bone marrow. Due to the difficulty in sampling and detection, few techniques exist to efficiently and consistently detect and quantify disseminated tumor cells (DTCs) in the bone marrow of cancer patients. Because mouse models represent a crucial tool with which to study cancer metastasis, we developed a novel method for the simple selection-free detection and quantification of bone marrow DTCs in mice. We have used this protocol to detect human and murine DTCs in xenograft, syngeneic, and genetically engineered mouse models. We are able to detect and quantify bone marrow DTCs in mice that do not have overt bone metastasis. This protocol is amenable not only for detection and quantification purposes but also to study the expression of markers of numerous biological processes or tissue-specificity.
骨转移是一种致命且无法治愈的疾病。它是癌细胞扩散至骨髓的结果。由于采样和检测存在困难，几乎没有技术能够有效且持续地检测和量化癌症患者骨髓中播散的肿瘤细胞（DTCs）。因为小鼠模型是研究癌症转移的关键工具，所以我们开发了一种新方法，用于简单、无需筛选地检测和量化小鼠骨髓中的DTCs。我们已使用该方案在异种移植、同基因和基因工程小鼠模型中检测人类和小鼠的DTCs。我们能够在没有明显骨转移的小鼠中检测和量化骨髓DTCs。该方案不仅适用于检测和量化目的，还可用于研究众多生物学过程或组织特异性标志物的表达。
