• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

猪流行性腹泻病毒核蛋白通过与C/EBP-β相互作用促进高迁移率族蛋白B1（HMGB1）的转录和释放。

Porcine epidemic diarrhea virus nucleoprotein contributes to HMGB1 transcription and release by interacting with C/EBP-β.

作者信息

Huan Chang-Chao, Wang Hua-Xia, Sheng Xiang-Xiang, Wang Rui, Wang Xin, Liao Ying, Liu Qin-Fang, Tong Guang-Zhi, Ding Chan, Fan Hong-Jie, Wu Jia-Qiang, Mao Xiang

机构信息

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China, 210095.

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, 200241.

出版信息

Oncotarget. 2016 Nov 15;7(46):75064-75080. doi: 10.18632/oncotarget.11991.

DOI:10.18632/oncotarget.11991
PMID:27634894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5342723/
Abstract

Porcine epidemic diarrhea is a devastating swine enteric disease, which is caused by porcine epidemic diarrhea virus (PEDV) infection. Our studies demonstrated that PEDV infection resulted in the up-regulation of proinflammatory cytokines. Meanwhile, PEDV infection and overexpression of viral nucleoprotein resulted in the acetylation and release of high mobility group box 1 proteins in vitro, an important proinflammatory response mediator, which contributes to the pathogenesis of various inflammatory diseases. Our studies also showed that SIRT1, histone acetyltransferase, and NF-κB regulated the acetylation and release of HMGB1. Chromatin immunoprecipitation, dual-luciferase reporter gene assay, and co-immunoprecipitation experiments illustrated that PEDV-N could induce HMGB1 transcription by interacting with C/EBP-β, which could bind to C/EBP motif in HMGB1 promotor region. Collectively, our data indicate PEDV-N contributes to HMGB1 transcription and the subsequent release/acetylation of HMGB1 during PEDV infection.

摘要

猪流行性腹泻是一种毁灭性的猪肠道疾病，由猪流行性腹泻病毒（PEDV）感染引起。我们的研究表明，PEDV感染导致促炎细胞因子上调。同时，PEDV感染和病毒核蛋白的过表达导致体外高迁移率族蛋白B1（HMGB1）的乙酰化和释放，HMGB1是一种重要的促炎反应介质，它参与各种炎症性疾病的发病机制。我们的研究还表明，沉默调节蛋白1（SIRT1）、组蛋白乙酰转移酶和核因子κB（NF-κB）调节HMGB1的乙酰化和释放。染色质免疫沉淀、双荧光素酶报告基因检测和免疫共沉淀实验表明，PEDV核蛋白（PEDV-N）可通过与C/EBP-β相互作用诱导HMGB1转录，C/EBP-β可与HMGB1启动子区域的C/EBP基序结合。总体而言，我们的数据表明，在PEDV感染期间，PEDV-N有助于HMGB1转录以及随后HMGB1的释放/乙酰化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/7e7ce38e3919/oncotarget-07-75064-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/61d315a56eb8/oncotarget-07-75064-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/409a0f288c80/oncotarget-07-75064-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/c1a809d1ea7f/oncotarget-07-75064-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/03d78d6e5c73/oncotarget-07-75064-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/1f0289289212/oncotarget-07-75064-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/ae08bebdfbf2/oncotarget-07-75064-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/7e7ce38e3919/oncotarget-07-75064-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/61d315a56eb8/oncotarget-07-75064-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/409a0f288c80/oncotarget-07-75064-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/c1a809d1ea7f/oncotarget-07-75064-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/03d78d6e5c73/oncotarget-07-75064-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/1f0289289212/oncotarget-07-75064-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/ae08bebdfbf2/oncotarget-07-75064-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90d8/5342723/7e7ce38e3919/oncotarget-07-75064-g007.jpg

相似文献

1
Porcine epidemic diarrhea virus nucleoprotein contributes to HMGB1 transcription and release by interacting with C/EBP-β.猪流行性腹泻病毒核蛋白通过与C/EBP-β相互作用促进高迁移率族蛋白B1（HMGB1）的转录和释放。
Oncotarget. 2016 Nov 15;7(46):75064-75080. doi: 10.18632/oncotarget.11991.
2
Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-β production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway.猪流行性腹泻病毒通过阻断RIG-I介导的途径抑制猪肠道上皮细胞中双链RNA诱导的干扰素-β产生。
Virol J. 2015 Aug 18;12:127. doi: 10.1186/s12985-015-0345-x.
3
MicroRNA-221-5p Inhibits Porcine Epidemic Diarrhea Virus Replication by Targeting Genomic Viral RNA and Activating the NF-κB Pathway.微小 RNA-221-5p 通过靶向基因组病毒 RNA 并激活 NF-κB 通路抑制猪流行性腹泻病毒复制。
Int J Mol Sci. 2018 Oct 29;19(11):3381. doi: 10.3390/ijms19113381.
4
Porcine epidemic diarrhea virus nsp4 induces pro-inflammatory cytokine and chemokine expression inhibiting viral replication in vitro.猪流行性腹泻病毒nsp4在体外诱导促炎细胞因子和趋化因子表达并抑制病毒复制。
Arch Virol. 2019 Apr;164(4):1147-1157. doi: 10.1007/s00705-019-04176-2. Epub 2019 Feb 24.
5
Glycyrrhizin Inhibits PEDV Infection and Proinflammatory Cytokine Secretion via the HMGB1/TLR4-MAPK p38 Pathway.甘草酸通过 HMGB1/TLR4-MAPK p38 通路抑制 PEDV 感染和促炎细胞因子的分泌。
Int J Mol Sci. 2020 Apr 23;21(8):2961. doi: 10.3390/ijms21082961.
6
Glycyrrhizin inhibits porcine epidemic diarrhea virus infection and attenuates the proinflammatory responses by inhibition of high mobility group box-1 protein.甘草酸抑制猪流行性腹泻病毒感染，并通过抑制高迁移率族蛋白盒1减轻促炎反应。
Arch Virol. 2017 Jun;162(6):1467-1476. doi: 10.1007/s00705-017-3259-7. Epub 2017 Feb 7.
7
Down-regulating heat shock protein 27 is involved in porcine epidemic diarrhea virus escaping from host antiviral mechanism.下调热休克蛋白27参与猪流行性腹泻病毒逃避宿主抗病毒机制。
Vet Microbiol. 2017 Jun;205:6-13. doi: 10.1016/j.vetmic.2017.04.031. Epub 2017 May 1.
8
Differential Protein Analysis of IPEC-J2 Cells Infected with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strains Elucidates the Pathogenesis of Infection.感染猪流行性腹泻病毒大流行株和经典株的IPEC-J2细胞的差异蛋白质分析阐明了感染的发病机制。
J Proteome Res. 2017 Jun 2;16(6):2113-2120. doi: 10.1021/acs.jproteome.6b00957. Epub 2017 May 18.
9
Suppression of Virulent Porcine Epidemic Diarrhea Virus Proliferation by the PI3K/Akt/GSK-3α/β Pathway.PI3K/Akt/GSK-3α/β信号通路对猪流行性腹泻病毒强毒株增殖的抑制作用
PLoS One. 2016 Aug 25;11(8):e0161508. doi: 10.1371/journal.pone.0161508. eCollection 2016.
10
Porcine Epidemic Diarrhea Virus Induces Autophagy to Benefit Its Replication.猪流行性腹泻病毒诱导自噬以利于其复制。
Viruses. 2017 Mar 19;9(3):53. doi: 10.3390/v9030053.

引用本文的文献

1
Traditional Chinese medicine as a promising choice for future control of PEDV.传统中医作为未来控制猪流行性腹泻病毒的一个有前景的选择。
Virus Res. 2025 Jun;356:199572. doi: 10.1016/j.virusres.2025.199572. Epub 2025 Apr 10.
2
In Vitro and In Vivo Evaluation of Strains as Prophylactic Agents Against Porcine Epidemic Diarrhea Virus.作为预防猪流行性腹泻病毒的菌株的体外和体内评价
Animals (Basel). 2025 Feb 7;15(4):470. doi: 10.3390/ani15040470.
3
Developing Next-Generation Live Attenuated Vaccines for Porcine Epidemic Diarrhea Using Reverse Genetic Techniques.

本文引用的文献

1
HMGB1 Promotes Hepatitis C Virus Replication by Interaction with Stem-Loop 4 in the Viral 5' Untranslated Region.高迁移率族蛋白B1通过与丙型肝炎病毒5'非翻译区的茎环4相互作用促进病毒复制。
J Virol. 2015 Dec 9;90(5):2332-44. doi: 10.1128/JVI.02795-15.
2
Outbreak of Porcine Epidemic Diarrhea Virus in Portugal, 2015.2015年葡萄牙猪流行性腹泻病毒疫情
Transbound Emerg Dis. 2015 Dec;62(6):586-8. doi: 10.1111/tbed.12409. Epub 2015 Sep 7.
3
Emergence of porcine epidemic diarrhea virus in southern Germany.德国南部猪流行性腹泻病毒的出现。
利用反向遗传技术研发猪流行性腹泻下一代减毒活疫苗。
Vaccines (Basel). 2024 May 19;12(5):557. doi: 10.3390/vaccines12050557.
4
The Network of Interactions between the Porcine Epidemic Diarrhea Virus Nucleocapsid and Host Cellular Proteins.猪流行性腹泻病毒核衣壳与宿主细胞蛋白间的相互作用网络。
Viruses. 2022 Oct 16;14(10):2269. doi: 10.3390/v14102269.
5
LncRNA OIP5-AS1 facilitates ox-LDL-induced endothelial cell injury through the miR-98-5p/HMGB1 axis.长链非编码RNA OIP5-AS1通过miR-98-5p/HMGB1轴促进氧化型低密度脂蛋白诱导的内皮细胞损伤。
Mol Cell Biochem. 2021 Jan;476(1):443-455. doi: 10.1007/s11010-020-03921-5. Epub 2020 Sep 29.
6
Glycyrrhizin: An alternative drug for the treatment of COVID-19 infection and the associated respiratory syndrome?甘草酸：治疗 COVID-19 感染及相关呼吸综合征的替代药物？
Pharmacol Ther. 2020 Oct;214:107618. doi: 10.1016/j.pharmthera.2020.107618. Epub 2020 Jun 24.
7
Glycyrrhizin Inhibits PEDV Infection and Proinflammatory Cytokine Secretion via the HMGB1/TLR4-MAPK p38 Pathway.甘草酸通过 HMGB1/TLR4-MAPK p38 通路抑制 PEDV 感染和促炎细胞因子的分泌。
Int J Mol Sci. 2020 Apr 23;21(8):2961. doi: 10.3390/ijms21082961.
8
Circular RNA CircEZH2 Suppresses Transmissible Gastroenteritis Coronavirus-induced Opening of Mitochondrial Permeability Transition Pore via Targeting MiR-22 in IPEC-J2.环状 RNA CircEZH2 通过靶向 IPEC-J2 中的 miR-22 抑制传染性胃肠炎冠状病毒诱导的线粒体通透性转换孔开放。
Int J Biol Sci. 2019 Jul 25;15(10):2051-2064. doi: 10.7150/ijbs.36532. eCollection 2019.
9
Differentially expressed non-coding RNAs induced by transmissible gastroenteritis virus potentially regulate inflammation and NF-κB pathway in porcine intestinal epithelial cell line.传染性胃肠炎病毒诱导的差异表达非编码 RNA 可能调节猪肠上皮细胞系中的炎症和 NF-κB 通路。
BMC Genomics. 2018 Oct 12;19(1):747. doi: 10.1186/s12864-018-5128-5.
10
An optimized HMGB1 expressed by recombinant rabies virus enhances immunogenicity through activation of dendritic cells in mice.重组狂犬病病毒表达的优化型高迁移率族蛋白B1通过激活小鼠树突状细胞增强免疫原性。
Oncotarget. 2017 Jun 5;8(48):83539-83554. doi: 10.18632/oncotarget.18368. eCollection 2017 Oct 13.
BMC Vet Res. 2015 Jul 2;11:142. doi: 10.1186/s12917-015-0454-1.
4
PARP-1 mediates LPS-induced HMGB1 release by macrophages through regulation of HMGB1 acetylation.聚（ADP-核糖）聚合酶-1（PARP-1）通过调节高迁移率族蛋白B1（HMGB1）的乙酰化作用，介导脂多糖（LPS）诱导的巨噬细胞HMGB1释放。
J Immunol. 2014 Dec 15;193(12):6114-23. doi: 10.4049/jimmunol.1400359. Epub 2014 Nov 12.
5
Porcine reproductive and respiratory syndrome virus infection triggers HMGB1 release to promote inflammatory cytokine production.猪繁殖与呼吸综合征病毒感染引发高迁移率族蛋白B1释放，以促进炎性细胞因子的产生。
Virology. 2014 Nov;468-470:1-9. doi: 10.1016/j.virol.2014.07.046. Epub 2014 Aug 16.
6
Microbe- and danger-induced inflammation.微生物和危险诱导的炎症
Mol Immunol. 2015 Feb;63(2):127-33. doi: 10.1016/j.molimm.2014.06.037. Epub 2014 Jul 16.
7
The compromise of macrophage functions by hyperoxia is attenuated by ethacrynic acid via inhibition of NF-κB-mediated release of high-mobility group box-1.依他尼酸通过抑制核因子κB介导的高迁移率族蛋白盒1释放，减轻高氧对巨噬细胞功能的损害。
Am J Respir Cell Mol Biol. 2015 Feb;52(2):171-82. doi: 10.1165/rcmb.2013-0544OC.
8
Interactions between the influenza A virus RNA polymerase components and retinoic acid-inducible gene I.甲型流感病毒RNA聚合酶组分与视黄酸诱导基因I之间的相互作用
J Virol. 2014 Sep;88(18):10432-47. doi: 10.1128/JVI.01383-14. Epub 2014 Jun 18.
9
High-mobility group box 1 is a novel deacetylation target of Sirtuin1.高迁移率族蛋白盒1是沉默调节蛋白1的一个新的去乙酰化靶点。
Kidney Int. 2015 Jan;87(1):95-108. doi: 10.1038/ki.2014.217. Epub 2014 Jun 18.
10
Porcine epidemic diarrhea virus nucleocapsid protein antagonizes beta interferon production by sequestering the interaction between IRF3 and TBK1.猪流行性腹泻病毒核衣壳蛋白通过隔离 IRF3 和 TBK1 之间的相互作用来拮抗β干扰素的产生。
J Virol. 2014 Aug;88(16):8936-45. doi: 10.1128/JVI.00700-14. Epub 2014 May 28.