Song Liqiang, Lu Yuqing, Zhang Jinpeng, Pan Cuili, Yang Xinming, Li Xiuquan, Liu Weihua, Li Lihui
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Genome. 2016 Oct;59(10):840-850. doi: 10.1139/gen-2016-0065. Epub 2016 Jul 28.
Agropyron cristatum (2n = 4x = 28, PPPP) is a wild relative of common wheat and confers several desirable agronomic traits to wheat, such as high grain number per spike and enhanced resistance to certain diseases. Development of wheat - A. cristatum 6P translocation lines facilitates its utilization in wheat improvement. In this study, 26 wheat - A. cristatum 6P translocation lines were characterized by in situ hybridization (ISH) and 6P-specific sequence-tagged-site (STS) markers. These translocation lines carried different 6P chromosomal segments, which covered the whole 6P chromosome. FISH results showed that 15, 5, and 6 lines were translocated onto wheat A, B, and D genomes, respectively. Compared with the previous reports, a fine physical map of 6P chromosome was constructed, consisting of 31 chromosomal bins with 255 STS markers. Twelve translocation lines containing 6PS13∼14 chromosomal bins were highly resistant to leaf rust. Two lines showed high grain number per spike, and three lines displayed both enhanced grain number per spike and thousand-grain weight. Development of wheat - A. cristatum 6P translocation lines will not only provide novel wheat germplasm for wheat breeding but also be helpful to broaden the genetic basis of common wheat.
冰草（2n = 4x = 28，PPPP）是普通小麦的野生近缘种，赋予小麦一些优良的农艺性状，如每穗粒数多和对某些病害的抗性增强。小麦-冰草6P易位系的培育有助于其在小麦改良中的利用。在本研究中，利用原位杂交（ISH）和6P特异性序列标签位点（STS）标记对26个小麦-冰草6P易位系进行了鉴定。这些易位系携带不同的6P染色体片段，覆盖了整个6P染色体。荧光原位杂交（FISH）结果表明，分别有15、5和6个品系易位到小麦的A、B和D基因组上。与之前的报道相比，构建了6P染色体的精细物理图谱，由31个染色体 bins 和255个 STS 标记组成。12个含有6PS13∼14染色体 bins 的易位系对叶锈病具有高度抗性。两个品系每穗粒数多，三个品系每穗粒数和千粒重均有所提高。小麦-冰草6P易位系的培育不仅为小麦育种提供了新的小麦种质，也有助于拓宽普通小麦的遗传基础。
