Oda Tomoyuki, Sakai Tadahiro, Hiraiwa Hideki, Hamada Takashi, Ono Yohei, Nakashima Motoshige, Ishizuka Shinya, Matsukawa Tetsuya, Yamashita Satoshi, Tsuchiya Saho, Ishiguro Naoki
Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
Biochem Biophys Res Commun. 2016 Oct 21;479(3):469-475. doi: 10.1016/j.bbrc.2016.09.085. Epub 2016 Sep 17.
The natural healing capacity of damaged articular cartilage is poor, rendering joint surface injuries a prime target for regenerative medicine. While autologous chondrocyte or mesenchymal stem cell (MSC) implantation can be applied to repair cartilage defects in young patients, no appropriate long-lasting treatment alternative is available for elderly patients with osteoarthritis (OA). Multipotent progenitor cells are reported to present in adult human articular cartilage, with a preponderance in OA cartilage. These facts led us to hypothesize the possible use of osteoarthritis-derived chondrocytes as a cell source for cartilage tissue engineering. We therefore analyzed chondrocyte- and stem cell-related markers, cell growth rate, and multipotency in OA chondrocytes (OACs) and bone marrow-derived MSCs, along with normal articular chondrocytes (ACs) as a control. OACs demonstrated similar phenotype and proliferation rate to MSCs. Furthermore, OACs exhibited multilineage differentiation ability with a greater chondrogenic differentiation ability than MSCs, which was equivalent to ACs. We conclude that chondrogenic capacity is not significantly affected by OA, and OACs could be a potential source of multipotent progenitor cells for cartilage tissue engineering.
受损关节软骨的自然愈合能力较差，这使得关节表面损伤成为再生医学的主要治疗对象。虽然自体软骨细胞或间充质干细胞（MSC）植入可用于修复年轻患者的软骨缺损，但对于患有骨关节炎（OA）的老年患者，尚无合适的长期治疗方案。据报道，多能祖细胞存在于成年人类关节软骨中，在OA软骨中更为常见。这些事实使我们推测，骨关节炎来源的软骨细胞可能作为软骨组织工程的细胞来源。因此，我们分析了OA软骨细胞（OAC）和骨髓来源的MSC中软骨细胞和干细胞相关标志物、细胞生长速率及多能性，并以正常关节软骨细胞（AC）作为对照。OAC表现出与MSC相似的表型和增殖速率。此外，OAC具有多谱系分化能力，其软骨形成分化能力比MSC更强，与AC相当。我们得出结论，软骨形成能力不会受到OA的显著影响，OAC可能是软骨组织工程中多能祖细胞的潜在来源。
