在BD MAX开放系统上开发并评估用于检测IMP、VIM和OXA-23碳青霉烯酶基因家族的多重实时PCR技术。
Development and evaluation of a multiplex real-time PCR for the detection of IMP, VIM, and OXA-23 carbapenemase gene families on the BD MAX open system.
作者信息
Teo Jeanette W P, La My-Van, Lin Raymond T P
机构信息
National University Hospital, Department of Laboratory Medicine, Division of Microbiology, Singapore, 119074, Republic of Singapore.
National Public Health Laboratory, Ministry of Health, 3 Biopolis Drive, Synapse #05-14/16, Singapore, 138623, Republic of Singapore.
出版信息
Diagn Microbiol Infect Dis. 2016 Dec;86(4):358-361. doi: 10.1016/j.diagmicrobio.2016.08.019. Epub 2016 Aug 26.
A multiplex real-time polymerase chain reaction (PCR) assay was developed for the detection of clinically prevalent IMP, VIM, and OXA-23 gene families. The assay was designed to work on the BD MAX open platform which is a fully automated system for all PCR processes including sample extraction to PCR resulting. A total of 107 well-characterized carbapenem resistant Enterobacteriaceae were evaluated and the results were 100% concordant with the reference test isolates. This assay will serve to complement PCR screens that detect the major carbapenemase families of NDM, KPC, and OXA-48-like.
开发了一种多重实时聚合酶链反应（PCR）检测方法，用于检测临床常见的IMP、VIM和OXA-23基因家族。该检测方法设计用于BD MAX开放平台，这是一个用于所有PCR过程的全自动系统，包括从样本提取到PCR结果分析。共评估了107株特征明确的耐碳青霉烯肠杆菌科细菌，结果与参考测试菌株100%一致。该检测方法将作为对检测NDM、KPC和OXA-48样等主要碳青霉烯酶家族的PCR筛查的补充。
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