胰腺囊肿液中作为生物标志物的DNA完整性
DNA integrity as biomarker in pancreatic cyst fluid.
作者信息
Utomo Wesley K, Janmaat Vincent T, Verhaar Auke P, Cros Jérôme, Lévy Philippe, Ruszniewski Philippe, den Berg Mirella S Vredenbregt-van, Jenster Guido, Bruno Marco J, Braat Henri, Fuhler Gwenny M, Peppelenbosch Maikel P
机构信息
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
Department de Pathology, Hôpital Beaujon, INSERM U1149, Université Paris Diderot Clichy, France.
出版信息
Am J Cancer Res. 2016 Aug 1;6(8):1837-41. eCollection 2016.
Identification of pancreatic cysts with malignant potential is important to prevent pancreatic cancer development. Integrity of cell free DNA (cfDNA) has been described as tumor biomarker, but its potential for pancreatic cancer is unclear. While normal apoptotic cells release uniformly truncated DNA, malignant tissues release long fragments of cell free DNA (cfDNA). We measured 247 base pair (bp) and 115 bp DNA fragments of ALU repeats by qPCR in serum from healthy controls and pancreatic cancer patients, and in cyst fluid from pancreatic cyst patients. No differences in total cfDNA (ALU115) and cfDNA integrity (ALU247/115) were observed between sera from healthy controls (n=19) and pancreatic cancer patients (n=19). Although elevated as compared to serum, but no differences in cfDNA were found in cyst fluid from high risk (n=10) and low risk (n=20) cyst patients. We conclude that cfDNA integrity is not a useful marker to identify (pre)malignant pancreatic lesions.
识别具有恶性潜能的胰腺囊肿对于预防胰腺癌的发生至关重要。游离DNA（cfDNA）的完整性已被描述为肿瘤生物标志物，但其在胰腺癌中的潜力尚不清楚。正常凋亡细胞释放均匀截断的DNA，而恶性组织释放游离DNA（cfDNA）的长片段。我们通过qPCR检测了健康对照者和胰腺癌患者血清以及胰腺囊肿患者囊液中ALU重复序列的247碱基对（bp）和115 bp DNA片段。在19名健康对照者和19名胰腺癌患者的血清中，未观察到总cfDNA（ALU115）和cfDNA完整性（ALU247/115）的差异。虽然与血清相比囊液中cfDNA升高，但在10名高风险和20名低风险囊肿患者的囊液中未发现cfDNA的差异。我们得出结论，cfDNA完整性不是识别（癌前）恶性胰腺病变的有用标志物。
Zotero 插件