• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

血浆DNA完整性作为结直肠癌化疗的预后生物标志物

Plasma DNA Integrity as a Prognostic Biomarker for Colorectal Cancer Chemotherapy.

作者信息

Zhu Feng, Ma Jing, Ru Dongdong, Wu Ningning, Zhang Yunhua, Li Huiyuan, Liu Xiaoli, Li Jianfeng, Zhang Huiling, Xu Yue, Zhao Jiangman, Tang Hui, Wang Yusheng, Fu Weihua

机构信息

Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.

Department of General Surgery, Jincheng People's Hospital, Jincheng 048026, China.

出版信息

J Oncol. 2021 May 26;2021:5569783. doi: 10.1155/2021/5569783. eCollection 2021.

DOI:10.1155/2021/5569783
PMID:34135960
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8175143/
Abstract

OBJECTIVES

To verify whether the concentrations and integrity index of circulating cell-free DNA (cfDNA) in serum may be clinically useful for the progression monitoring of colorectal cancer (CRC) patients.

METHODS

Serum samples were collected from 76 primary CRC patients who underwent surgery, including 60 with chemotherapy and 43 with follow-up. Long (247 bp) and short (115 bp) DNA fragments in serum were detected by real-time quantitative PCR by amplifying the ALU repeats. Ten serum traditional biomarkers levels were detected by chemiluminescence immunoassay assay.

RESULTS

The median DNA integrity index (ALU247/ALU115) of serum DNA in the preoperative group was significantly higher than those in the postchemotherapy and the follow-up groups, while cfDNA concentration (ALU115) was significantly lower in the preoperative group compared with the postchemotherapy and the follow-up groups. CEA and CA242 were significantly lower in the postoperative group than in the preoperative group.

CONCLUSIONS

Serum DNA integrity index (ALU247/115) may prove to be a promising candidate biomarker for prognostic prediction of CRC who underwent chemotherapy and during short-term follow-up.

摘要

目的

验证血清中循环游离DNA（cfDNA）的浓度和完整性指数是否可用于临床监测结直肠癌（CRC）患者的病情进展。

方法

收集76例接受手术的原发性CRC患者的血清样本，其中60例接受化疗，43例接受随访。通过实时定量PCR扩增ALU重复序列，检测血清中的长（247bp）和短（115bp）DNA片段。采用化学发光免疫分析法检测10种血清传统生物标志物水平。

结果

术前组血清DNA的中位DNA完整性指数（ALU247/ALU115）显著高于化疗后组和随访组，而术前组的cfDNA浓度（ALU115）显著低于化疗后组和随访组。术后组的CEA和CA242显著低于术前组。

结论

血清DNA完整性指数（ALU247/115）可能是预测接受化疗的CRC患者预后及短期随访的有前景的候选生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/775e2acadf05/JO2021-5569783.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/a36290794a0d/JO2021-5569783.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/d20deca5ea25/JO2021-5569783.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/a346068707c1/JO2021-5569783.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/40ca9878fa54/JO2021-5569783.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/fbfdc240b63f/JO2021-5569783.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/386d808f2034/JO2021-5569783.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/8569519d2f29/JO2021-5569783.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/7e448ac33247/JO2021-5569783.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/775e2acadf05/JO2021-5569783.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/a36290794a0d/JO2021-5569783.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/d20deca5ea25/JO2021-5569783.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/a346068707c1/JO2021-5569783.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/40ca9878fa54/JO2021-5569783.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/fbfdc240b63f/JO2021-5569783.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/386d808f2034/JO2021-5569783.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/8569519d2f29/JO2021-5569783.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/7e448ac33247/JO2021-5569783.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6907/8175143/775e2acadf05/JO2021-5569783.009.jpg

相似文献

1
Plasma DNA Integrity as a Prognostic Biomarker for Colorectal Cancer Chemotherapy.血浆DNA完整性作为结直肠癌化疗的预后生物标志物
J Oncol. 2021 May 26;2021:5569783. doi: 10.1155/2021/5569783. eCollection 2021.
2
Circulating cell-free DNA in serum as a biomarker for diagnosis and prognostic prediction of colorectal cancer.血清中循环游离DNA作为结直肠癌诊断和预后预测的生物标志物。
Br J Cancer. 2014 Oct 14;111(8):1482-9. doi: 10.1038/bjc.2014.470. Epub 2014 Aug 26.
3
[Value of the concentration and integrity of serum cell-free DNA for the clinical diagnosis of esophageal carcinoma].[血清游离DNA浓度及完整性在食管癌临床诊断中的价值]
Zhonghua Zhong Liu Za Zhi. 2018 Dec 23;40(12):905-910. doi: 10.3760/cma.j.issn.0253-3766.2018.12.006.
4
Investigation of Efficacy Evaluation Comparison of cfDNA and CEA in Colorectal Cancer.循环游离DNA与癌胚抗原在结直肠癌疗效评估中的比较研究
Clin Lab. 2016 Oct 1;62(10):1947-1953. doi: 10.7754/Clin.Lab.2016.160215.
5
The level and integrity of plasma circulating cell-free DNA in patients with primary multiple myeloma.原发性多发性骨髓瘤患者血浆循环游离DNA的水平及完整性
Transl Cancer Res. 2022 Nov;11(11):4137-4147. doi: 10.21037/tcr-22-2416.
6
Cell-free circulating ALU repeats in serum have a prognostic value for colorectal cancer patients.血清中无细胞游离的 ALU 重复序列对结直肠癌患者具有预后价值。
Cancer Biomark. 2023;37(4):237-248. doi: 10.3233/CBM-210536.
7
Circulating cell-free DNA: a promising marker of regional lymphonode metastasis in breast cancer patients.循环游离 DNA：乳腺癌患者区域淋巴结转移的有前途的标志物。
Cancer Biomark. 2012;11(2-3):89-98. doi: 10.3233/CBM-2012-0263.
8
Circulating cell-free DNA in serum as a biomarker of colorectal cancer.血清中循环无细胞 DNA 作为结直肠癌的生物标志物。
J Clin Pathol. 2013 Sep;66(9):775-8. doi: 10.1136/jclinpath-2013-201521. Epub 2013 Jul 6.
9
Circulating cell-free DNA in plasma of colorectal cancer patients - A potential biomarker for tumor burden.结直肠癌患者血浆中循环游离DNA——一种肿瘤负荷的潜在生物标志物。
Surg Oncol. 2017 Dec;26(4):395-401. doi: 10.1016/j.suronc.2017.08.001. Epub 2017 Aug 7.
10
DNA integrity as biomarker in pancreatic cyst fluid.胰腺囊肿液中作为生物标志物的DNA完整性
Am J Cancer Res. 2016 Aug 1;6(8):1837-41. eCollection 2016.

引用本文的文献

1
Application of plasma cell-free DNA in screening of advanced colorectal adenoma.浆细胞游离DNA在晚期结直肠腺瘤筛查中的应用
Eur J Med Res. 2025 Feb 25;30(1):136. doi: 10.1186/s40001-025-02313-z.
2
Correlation between plasma ccfDNA, mtDNA changes, CTCs, and epithelial-mesenchymal transition in breast cancer patients undergoing NACT.接受新辅助化疗的乳腺癌患者血浆 ccfDNA、mtDNA 变化、CTC 与上皮间质转化的相关性。
Turk J Med Sci. 2024 Mar 11;54(4):652-665. doi: 10.55730/1300-0144.5834. eCollection 2024.
3
Diagnostic value of combined detection of plasma cfDNA concentration and integrity in NSCLC.

本文引用的文献

1
Clinicopathological features and prognosis of AFP-producing colorectal cancer: a single-center analysis of 20 cases.产甲胎蛋白的结直肠癌的临床病理特征及预后：20例单中心分析
Cancer Manag Res. 2019 May 16;11:4557-4567. doi: 10.2147/CMAR.S196919. eCollection 2019.
2
CA242 as a biomarker for pancreatic cancer and other diseases.CA242 作为胰腺癌和其他疾病的生物标志物。
Prog Mol Biol Transl Sci. 2019;162:229-239. doi: 10.1016/bs.pmbts.2018.12.007. Epub 2019 Mar 6.
3
Colorectal Cancer Incidence Trends by Age, Stage, and Racial/Ethnic Group in California, 1990-2014.
血浆游离DNA浓度与完整性联合检测在非小细胞肺癌中的诊断价值
Lung Cancer Manag. 2024 May 16;13(1):LMT64. doi: 10.2217/lmt-2023-0009. eCollection 2024.
4
Mutation Analysis of Pancreatic Juice and Plasma for the Detection of Pancreatic Cancer.胰腺液和血浆的突变分析用于胰腺癌的检测。
Int J Mol Sci. 2023 Aug 23;24(17):13116. doi: 10.3390/ijms241713116.
5
Estimation of ALU Repetitive Elements in Plasma as a Cost-Effective Liquid Biopsy Tool for Disease Prognosis in Breast Cancer.评估血浆中的ALU重复元件作为一种具有成本效益的液体活检工具用于乳腺癌疾病预后评估
Cancers (Basel). 2023 Feb 7;15(4):1054. doi: 10.3390/cancers15041054.
6
The Utility of Repetitive Cell-Free DNA in Cancer Liquid Biopsies.循环游离DNA在癌症液体活检中的应用
Diagnostics (Basel). 2022 Jun 1;12(6):1363. doi: 10.3390/diagnostics12061363.
7
ALU repeat as potential molecular marker in the detection and prognosis of different cancer types: A systematic review.ALU重复序列作为不同癌症类型检测和预后的潜在分子标志物：一项系统综述。
Mol Clin Oncol. 2022 Apr;16(4):86. doi: 10.3892/mco.2022.2519. Epub 2022 Feb 21.
加利福尼亚州 1990-2014 年按年龄、分期和种族/族裔划分的结直肠癌发病率趋势
Cancer Epidemiol Biomarkers Prev. 2018 Sep;27(9):1011-1018. doi: 10.1158/1055-9965.EPI-18-0030. Epub 2018 Aug 16.
4
Serum CA125 concentration as a predictor of peritoneal dissemination of colorectal cancer in men and women.血清CA125浓度作为男性和女性结直肠癌腹膜播散的预测指标。
Medicine (Baltimore). 2016 Nov;95(47):e5177. doi: 10.1097/MD.0000000000005177.
5
Epidemiological Trends in Colorectal Cancer in China: An Ecological Study.中国结直肠癌的流行病学趋势：一项生态学研究。
Dig Dis Sci. 2017 Jan;62(1):235-243. doi: 10.1007/s10620-016-4362-4. Epub 2016 Oct 31.
6
Free/Total Serum Prostate-Specific Antigen Ratio in Women with Colorectal Cancer Has Prognostic Significance.结直肠癌女性患者血清游离/总前列腺特异性抗原比值具有预后意义。
J Gastrointest Cancer. 2017 Mar;48(1):8-12. doi: 10.1007/s12029-016-9859-8.
7
Molecularly determined total tumour load in lymph nodes of stage I-II colon cancer patients correlates with high-risk factors. A multicentre prospective study.I-II期结肠癌患者淋巴结中分子测定的总肿瘤负荷与高危因素相关。一项多中心前瞻性研究。
Virchows Arch. 2016 Oct;469(4):385-94. doi: 10.1007/s00428-016-1990-1. Epub 2016 Jul 22.
8
Circulating free DNA concentration is an independent prognostic biomarker in lung cancer.循环游离 DNA 浓度是肺癌的独立预后生物标志物。
Eur Respir J. 2015 Dec;46(6):1773-80. doi: 10.1183/13993003.00676-2015. Epub 2015 Oct 22.
9
Cell-free DNA levels in plasma of patients with non-small-cell lung cancer and inflammatory lung disease.非小细胞肺癌和炎性肺病患者血浆中的游离DNA水平
Br J Cancer. 2015 Jul 28;113(3):476-83. doi: 10.1038/bjc.2015.225. Epub 2015 Jun 30.
10
Role of blood tumor markers in predicting metastasis and local recurrence after curative resection of colon cancer.血液肿瘤标志物在预测结肠癌根治性切除术后转移和局部复发中的作用。
Int J Clin Exp Med. 2015 Jan 15;8(1):982-90. eCollection 2015.