Mascarenhas Rodrigo O, Prezotto Leandro F, Perondini André Luiz P, Marino Celso Luiz, Selivon Denise
Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
Departamento de Genética, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Genet Mol Biol. 2016 Oct-Dec;39(4):600-610. doi: 10.1590/1678-4685-GMB-2016-0075. Epub 2016 Sep 19.
The endosymbiont Wolbachia is efficiently transmitted from females to their progenies, but horizontal transmission between different taxa is also known to occur. Aiming to determine if horizontal transmission might have occurred between Anastrepha fruit flies and associated braconid wasps, infection by Wolbachia was screened by amplification of a fragment of the wsp gene. Eight species of the genus Anastrepha were analyzed, from which six species of associated parasitoid wasps were recovered. The endosymbiont was found in seven Anastrepha species and in five species of braconids. The WSP Typing methodology detected eight wsp alleles belonging to Wolbachia supergroup A. Three were already known and five were new ones, among which four were found to be putative recombinant haplotypes. Two samples of Anastrepha obliqua and one sample of Doryctobracon brasiliensis showed multiple infection. Single infection by Wolbachia was found in the majority of samples. The distribution of Wolbachia harboring distinct alleles differed significantly between fruit flies and wasps. However, in nine samples of fruit flies and associated wasps, Wolbachia harbored the same wsp allele. These congruences suggest that horizontal transfer of Wolbachia might have occurred in the communities of fruit flies and their braconid parasitoids.
内共生菌沃尔巴克氏体能够有效地从雌性传递到其后代，但不同分类群之间的水平传播也已为人所知。为了确定在按实蝇属果蝇与其相关的茧蜂之间是否可能发生了水平传播，通过扩增 wsp 基因片段来筛选沃尔巴克氏体的感染情况。分析了按实蝇属的 8 个物种，从中回收了 6 种相关的寄生蜂。在内共生菌在 7 种按实蝇和 5 种茧蜂中被发现。WSP 分型方法检测到属于沃尔巴克氏体 A 超群的 8 个 wsp 等位基因。其中 3 个是已知的，5 个是新的，其中 4 个被发现是推定的重组单倍型。两个斜纹按实蝇样本和一个巴西潜蝇茧蜂样本显示出多重感染。在大多数样本中发现了沃尔巴克氏体的单一感染。携带不同等位基因的沃尔巴克氏体在果蝇和黄蜂之间的分布存在显著差异。然而，在 9 个果蝇及其相关黄蜂样本中，沃尔巴克氏体携带相同的 wsp 等位基因。这些一致性表明，沃尔巴克氏体可能在果蝇及其茧蜂寄生蜂群落中发生了水平转移。
