单侧肾切除大鼠的血浆可刺激肾皮质切片中的磷脂甲基化和花生四烯酸释放。
Plasma from uninephrectomized rats stimulates phospholipid methylation and arachidonic acid release in renal-cortical slices.
作者信息
Banfić H, Gatalica Z
机构信息
Department of Physiology, Faculty of Medicine, University of Zagreb, Yugoslavia.
出版信息
Biochem J. 1989 Jun 1;260(2):365-9. doi: 10.1042/bj2600365.
Phospholipid methylation and arachidonic acid release in renal-cortical slices was investigated in vitro after addition of plasma from uninephrectomized or sham-operated rats. Plasma from uninephrectomized rats ('uni-plasma') stimulated phospholipid methylation when obtained within the first 3 h after uninephrectomy. With different amounts of added plasma a graded response in phospholipid methylation was obtained. Addition of 50 nM-12-O-tetradecanoylphorbol 13-acetate for 10 min to intact slices also stimulated phospholipid methylation, whereas incubation of slices before addition of 'uni-plasma' with 100 microM-1-(5-isoquinolinylsulphonyl)-2-methylpiperazine prevented it, suggesting that protein kinase C stimulates phospholipid methylation in renal-cortical slices. Plasma from uninephrectomized rats also stimulates [3H]arachidonic acid release from phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) via activation of phospholipase A2. Two mechanisms of phospholipase A2 activation are proposed: first, in which it is activated by protein kinase C and releases 3H radioactivity from PtdCho, and second, in which phospholipase A2 is stimulated by Ca2+ ions and releases 3H radioactivity from PtdEtn.
在添加来自单侧肾切除或假手术大鼠的血浆后，对肾皮质切片中的磷脂甲基化和花生四烯酸释放进行了体外研究。单侧肾切除大鼠的血浆（“单侧血浆”）在单侧肾切除后的前3小时内获取时可刺激磷脂甲基化。添加不同量的血浆可获得磷脂甲基化的分级反应。向完整切片中添加50 nM - 12 - O - 十四烷酰佛波醇13 - 乙酸酯10分钟也可刺激磷脂甲基化，而在添加“单侧血浆”之前用100 microM - 1 -（5 - 异喹啉磺酰基）- 2 - 甲基哌嗪孵育切片可阻止这种情况，这表明蛋白激酶C刺激肾皮质切片中的磷脂甲基化。单侧肾切除大鼠的血浆还通过激活磷脂酶A2刺激[3H]花生四烯酸从磷脂酰胆碱（PtdCho）和磷脂酰乙醇胺（PtdEtn）中释放。提出了磷脂酶A2激活的两种机制：第一种，它被蛋白激酶C激活并从PtdCho释放3H放射性；第二种，磷脂酶A2被Ca2 +离子刺激并从PtdEtn释放3H放射性。
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