链霉菌K15 D-丙氨酰-D-丙氨酸羧肽酶/青霉素结合蛋白。活性位点及N端区域序列。
The Streptomyces K15 DD-peptidase/penicillin-binding protein. Active site and sequence of the N-terminal region.
作者信息
Leyh-Bouille M, Van Beeumen J, Renier-Pirlot S, Joris B, Nguyen-Distèche M, Ghuysen J M
机构信息
Service de Microbiologie, Université de Liège, Institut de Chimie, Belgium.
出版信息
Biochem J. 1989 Jun 1;260(2):601-4. doi: 10.1042/bj2600601.
Abstract
The N-terminal region of the Streptomyces K15 DD-peptidase/penicillin-binding protein shows high homology with that of other penicillin-interactive proteins or domains. The active-site serine residue of the conserved tetrad Ser-Xaa-Xaa-Lys occurs at position 35. There is no indication for the presence of a signal peptide or an N-terminal hydrophobic sequence, suggesting that the Streptomyces K15 enzyme is probably anchored to the membrane by a C-terminal peptide segment.
摘要
链霉菌K15 DD-肽酶/青霉素结合蛋白的N端区域与其他青霉素相互作用蛋白或结构域的N端区域具有高度同源性。保守的四联体Ser-Xaa-Xaa-Lys中的活性位点丝氨酸残基位于第35位。没有迹象表明存在信号肽或N端疏水序列，这表明链霉菌K15酶可能通过C端肽段锚定在膜上。
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引用本文的文献
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Amino acid sequence of the penicillin-binding protein/DD-peptidase of Streptomyces K15. Predicted secondary structures of the low Mr penicillin-binding proteins of class A.
Biochem J. 1991 Oct 1;279 ( Pt 1)(Pt 1):223-30. doi: 10.1042/bj2790223.
本文引用的文献
1
The penicillin-binding site in the exocellular DD-carboxypeptidase-transpeptidase of Actinomadura R39.
Biochem J. 1981 Jan 1;193(1):83-6. doi: 10.1042/bj1930083.
2
Isolation of the membrane-bound 26 000-Mr penicillin-binding protein of Streptomyces strain K15 in the form of a penicillin-sensitive D-alanyl-D-alanine-cleaving transpeptidase.
Biochem J. 1982 Oct 1;207(1):109-15. doi: 10.1042/bj2070109.
3
Analysis of phenylthiohydantoins by ultrasensitive gradient high-performance liquid chromatography.
Methods Enzymol. 1983;91:486-93. doi: 10.1016/s0076-6879(83)91045-5.
4
ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4897-901. doi: 10.1073/pnas.78.8.4897.
5
On the process of cellular division in Escherichia coli: nucleotide sequence of the gene for penicillin-binding protein 3.
Mol Gen Genet. 1983;191(1):1-9. doi: 10.1007/BF00330881.
6
Production of thiol-penicillin-binding protein 3 of Escherichia coli using a two primer method of site-directed mutagenesis.
EMBO J. 1985 Jan;4(1):231-5. doi: 10.1002/j.1460-2075.1985.tb02340.x.
7
Sequence of the OXA2 beta-lactamase: comparison with other penicillin-reactive enzymes.
FEBS Lett. 1985 Oct 21;191(1):39-44. doi: 10.1016/0014-5793(85)80989-3.
8
Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene.
Eur J Biochem. 1987 Feb 2;162(3):509-18. doi: 10.1111/j.1432-1033.1987.tb10669.x.
9
Streptomyces K15 DD-peptidase-catalysed reactions with suicide beta-lactam carbonyl donors.
Biochem J. 1986 Apr 1;235(1):177-82. doi: 10.1042/bj2350177.
10
Effects of thiol reagents on Streptomyces K15 DD-peptidase-catalysed reactions.
Biochem J. 1987 Feb 1;241(3):893-7. doi: 10.1042/bj2410893.
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