具有三甲基鸟苷帽结构的前体信使核糖核酸在体外能够高效剪接。

Pre-mRNA with a trimethylguanosine cap structure can be spliced efficiently in vitro.

作者信息

Tokumoto Y, Iwase R, Sekine M, Hata T, Ohshima Y

机构信息

Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.

出版信息

Biochem Biophys Res Commun. 1989 Aug 15;162(3):977-83. doi: 10.1016/0006-291x(89)90769-9.

DOI:10.1016/0006-291x(89)90769-9

PMID:2764950

Abstract

A chemically synthesized 2,2,7-trimethylguanosine cap (TMG-cap) analogue was added to an in vitro pre-mRNA splicing system. A slight inhibition of the splicing was observed, but it was much less remarkable than that by a 7-methylguanosine cap analogue. This result does not favor a direct role of the TMG-cap structure of small nuclear RNAs in pre-mRNA splicing, and suggests that the TMG-cap does not effectively interact with the 7-methylguanosine cap binding factors. Using the SP6 in vitro transcription system and a TMG-cap analogue, we prepared a beta-globin transcript which has a TMG-cap at the 5' end. This TMG-capped transcript was spliced accurately and efficiently in vitro.

摘要

将化学合成的2,2,7-三甲基鸟苷帽（TMG帽）类似物添加到体外前体mRNA剪接系统中。观察到剪接有轻微抑制，但比7-甲基鸟苷帽类似物引起的抑制要小得多。该结果不支持小核RNA的TMG帽结构在前体mRNA剪接中起直接作用，并表明TMG帽不能有效地与7-甲基鸟苷帽结合因子相互作用。利用SP6体外转录系统和TMG帽类似物，我们制备了在5'端带有TMG帽的β-珠蛋白转录本。这种带有TMG帽的转录本在体外能准确且高效地进行剪接。