Wu Chiu-Yeh, Liang Zeng-Chin, Tseng Chin-Yin, Hu Shu-Hui
Department of Culinary Arts, Chung Chou University of Science and Technology, Changhua, Taiwan, R.O.C.
Department of Bioresources, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.).
Int J Med Mushrooms. 2016;18(7):589-97. doi: 10.1615/intjmedmushrooms.v18.i7.40.
We investigated the effects of light intensity in the 3 cultivation stages separately-the mycelium colonization stage, the primordial initiation stage, and the fruiting stage (in order)-on fruiting body and bioactive compound production by Cordyceps militaris. In the mycelium colonization stage, rice substrates were incubated in a spawn running room at 23°C. During the primordial initiation stage, C. militaris was grown at 18°C and illuminated 12 hours/day. In the fruiting stage the temperature was 23°C, with illumination provided 12 hours/day. The highest fruiting body yield and biological efficiency were 4.06 g dry weight/bottle and 86.83%, respectively, under 1750 ± 250 lux during the second and third stages. The cordycepin content was highest during the second and third stages under 1250 ± 250 lux. The mannitol and polysaccharide contents were highest under 1250 ± 250 and 1750 ± 250 lux during the primordial initiation stage and the fruiting stage, respectively. Thus, with controlled lighting, C. militaris can be cultivated in rice-water medium to increase fruiting body yield and bioactive compound production.
我们分别研究了光照强度在蛹虫草三个栽培阶段（依次为菌丝体定植阶段、原基形成阶段和子实体形成阶段）对其子实体产量和生物活性化合物产量的影响。在菌丝体定植阶段，将水稻基质在23°C的发菌室中培养。在原基形成阶段，蛹虫草在18°C下生长，每天光照12小时。在子实体形成阶段，温度为23°C，每天光照12小时。在第二和第三阶段，光照强度为1750±250勒克斯时，子实体产量最高，干重为4.06克/瓶，生物学效率分别为86.83%。在1250±250勒克斯下，第二和第三阶段的虫草素含量最高。在原基形成阶段和子实体形成阶段，光照强度分别为1250±250勒克斯和1750±250勒克斯时，甘露醇和多糖含量最高。因此，通过控制光照，蛹虫草可以在水稻-水培养基中进行栽培，以提高子实体产量和生物活性化合物产量。
