Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Biosensor Research Center, Endocrinology and Metabolism Molecular Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Chem Biol Drug Des. 2017 Apr;89(4):498-504. doi: 10.1111/cbdd.12871. Epub 2016 Nov 5.
Nanobodies are important biomolecules for tumor targeting. In this study, we synthesized and labeled anti-epidermal growth factor receptor (EGFR) nanobody OA-cb6 with Tc(CO) and evaluated its characteristics for targeting the EGFR in the A431 human epidermal carcinoma cell line. Nanobody radiolabeling was achieved with high yield and radiochemical purity, and the radioconjugate was stable. Biodistribution results in nude mice exhibited a favorable tumor-to-muscle ratio at 4-hr postinjection, and tumor location was visualized at 4 hr after injection of radiolabeled nanobody. Our result showed that the OA-cb6- Tc-tricarbonyl radiolabeled nanobody is a promising radiolabeled biomolecule for tumor imaging in cancers with high EGFR overexpression.
纳米抗体是用于肿瘤靶向的重要生物分子。在这项研究中，我们合成并标记了抗表皮生长因子受体（EGFR）纳米抗体 OA-cb6 的 Tc(CO)，并评估了其在 A431 人表皮癌细胞系中靶向 EGFR 的特性。纳米抗体的放射性标记实现了高产量和放射化学纯度，且放射性标记物稳定。裸鼠的体内分布结果显示，在注射后 4 小时，肿瘤与肌肉的比值良好，注射放射性标记纳米抗体后 4 小时即可观察到肿瘤位置。我们的结果表明，OA-cb6-Tc-三羰基标记的纳米抗体是一种很有前途的用于高 EGFR 过表达癌症的肿瘤成像放射性标记生物分子。
