一种使用小鼠脾细胞直接感染和分枝杆菌生长指示管（MGIT）系统的简化分枝杆菌生长抑制试验（MGIA）。
A simplified mycobacterial growth inhibition assay (MGIA) using direct infection of mouse splenocytes and the MGIT system.
作者信息
Yang Amy L, Schmidt Thomas E, Stibitz Scott, Derrick Steven C, Morris Sheldon L, Parra Marcela
机构信息
Center for Biologics Evaluation and Research, United States Food and Drug Administration, Silver Spring, MD, USA.
Center for Biologics Evaluation and Research, United States Food and Drug Administration, Silver Spring, MD, USA.
出版信息
J Microbiol Methods. 2016 Dec;131:7-9. doi: 10.1016/j.mimet.2016.09.010. Epub 2016 Sep 17.
We describe a simplified Mycobacterial Growth Inhibition Assay (MGIA) for pre-clinical assessment of vaccine-mediated protection in mice. The assay is accomplished by directly infecting splenocytes from vaccinated mice with Mycobacterium tuberculosis and quantifying mycobacteria using Mycobacterial Growth Indicator Tubes (MGIT). Vaccine-mediated immunogenicity detected by this assay correlated with protection.
我们描述了一种简化的分枝杆菌生长抑制试验（MGIA），用于在小鼠中进行疫苗介导的保护作用的临床前评估。该试验通过用结核分枝杆菌直接感染接种疫苗小鼠的脾细胞，并使用分枝杆菌生长指示管（MGIT）对分枝杆菌进行定量来完成。通过该试验检测到的疫苗介导的免疫原性与保护作用相关。
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