薄琼脂 7H11 用于分离结核分枝杆菌复合体的评估。
Evaluation of thin-layer agar 7H11 for the isolation of Mycobacterium tuberculosis complex.
机构信息
Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi, Pakistan.
出版信息
Int J Tuberc Lung Dis. 2010 Oct;14(10):1354-6.
We evaluated the thin-layer agar (TLA) method for the recovery of Mycobacterium tuberculosis complex and compared the results with the BACTEC Mycobacteria Growth Indicator Tube (MGIT) 960 system. A total of 53 mycobacterial isolates were isolated on both media. The recovery rates of mycobacteria on TLA and BACTEC MGIT 960 system were respectively 90.6% and 96.2%. Mean time to detection of mycobacteria on TLA was 12.5 compared to 11.2 days on BACTEC MGIT 960. TLA is a simple technique and can be used as an alternative to the Löwenstein-Jensen medium and BACTEC MGIT 960 for the isolation of mycobacteria in resource-poor settings.
我们评估了用于恢复结核分枝杆菌复合群的薄层琼脂（TLA）方法，并将结果与 BACTEC 分枝杆菌生长指示管（MGIT）960 系统进行了比较。两种培养基上共分离出 53 株分枝杆菌分离株。TLA 和 BACTEC MGIT 960 系统上分枝杆菌的回收率分别为 90.6%和 96.2%。TLA 检测分枝杆菌的平均时间为 12.5 天，而 BACTEC MGIT 960 为 11.2 天。TLA 是一种简单的技术，可作为 Löwenstein-Jensen 培养基和 BACTEC MGIT 960 的替代品，用于资源匮乏环境中分枝杆菌的分离。
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