Zhou Liting, Zheng Dongchun, Wang Shuyue, Zhu Jian, Jia Yiyang, Sun Di, Xu Jin, Wang Qi, Chen Huaiji, Xu Feng, Li Bo, Ye Lin
Department of Occupational and Environmental Health, School of Public Health, Jilin University, 1163 Xin Min Street, Changchun, 130021 China.
Department of Emergency, China-Japan Union Hospital, Jilin University, Changchun, China.
Springerplus. 2016 Sep 13;5(1):1533. doi: 10.1186/s40064-016-3177-2. eCollection 2016.
Toll-like receptor 4 (TLR4) is known to be involved in innate immunity and inflammatory responses that play important roles in the pathogenesis of coronary artery disease (CAD). But the relationship between TLR4 gene and CAD has yet to be investigated. The present study aimed to evaluate the association of TLR4 gene polymorphisms with CAD susceptibility in a Chinese Han population.
A total of 1094 subjects (577 unrelated patients with CAD and 517 controls) were recruited between 2008 and 2012. Three tag SNPs (rs1927907, rs1927911 and rs11536889) present in the TLR4 gene were genotyped using Sequenom Mass-ARRAY system.
The genotypic distributions of the three SNPs were not deviate from Hardy-Weinberg equilibrium. There was no significant difference in distributions of allelic frequencies of each SNPs between healthy controls and CAD patients (P > 0.05). Genotype frequencies of TLR4 gene did not show any statistically significant difference between the two groups in co-dominant, dominant or recessive genetic models (P > 0.05). The frequency of haplotypes in the case group was similar to that in the control group (P > 0.05).
TLR4 gene do not relate to genetic susceptibility of CAD in the Chinese Han population.
已知Toll样受体4（TLR4）参与先天性免疫和炎症反应，这些反应在冠状动脉疾病（CAD）的发病机制中起重要作用。但TLR4基因与CAD之间的关系尚未得到研究。本研究旨在评估中国汉族人群中TLR4基因多态性与CAD易感性的关联。
2008年至2012年期间共招募了1094名受试者（577名无亲缘关系的CAD患者和517名对照）。使用Sequenom Mass-ARRAY系统对TLR4基因中存在的三个标签单核苷酸多态性（rs1927907、rs1927911和rs11536889）进行基因分型。
这三个单核苷酸多态性的基因型分布均符合Hardy-Weinberg平衡。健康对照与CAD患者之间每个单核苷酸多态性的等位基因频率分布无显著差异（P>0.05）。在共显性、显性或隐性遗传模型中，两组之间TLR4基因的基因型频率均无统计学显著差异（P>0.05）。病例组的单倍型频率与对照组相似（P>0.05）。
在中国汉族人群中，TLR4基因与CAD的遗传易感性无关。
