aDivision of Gynaecology and Reproductive Medicine, Department of Gynaecology, Humanitas Fertility Center, Humanitas Research Hospital, Rozzano, Milan, Italy bDepartment of Obstetrics, Gynaecology and Reproductive Sciences, Yale University, School of Medicine, New Haven, Connecticut, USA cART Italian National Register, National Centre for Epidemiology, Surveillance and Health Promotion, National Health Institute, Rome, Italy.
Curr Opin Endocrinol Diabetes Obes. 2016 Dec;23(6):445-450. doi: 10.1097/MED.0000000000000289.
The purpose is to determine the efficiency and efficacy of oocyte cryopreservation by slow freezing versus vitrification, recent data collected from the Italian National Assisted Reproductive Technology Register during the period 2009-2014 will be presented and reviewed. The data on oocyte cryopreservation were also compared with the results obtained with embryo cryopreservation and relative IVF with fresh oocytes.
During the period 2009-2014 preservation of oocytes by vitrification had a significantly higher survival rate, implantation, and pregnancy rate than slow freezing; however, there are still large variations in success rates among centers in relation to the number of procedures performed.
Vitrification has now become the method of choice for oocyte cryopreservation because of better results than slow freezing, but still requires a more standardized utilization. The transfer of fresh or cryopreserved embryo still shows a statistically significant better performance than transfers with embryos obtained with cryopreserved oocytes. Only in a few centers with much experience in cryopreservation are the results between transfers of frozen embryos or embryos obtained from oocyte cryopreservation comparable.
目的是确定慢速冷冻与玻璃化冷冻对卵母细胞冷冻保存的效率和效果。将呈现并回顾 2009-2014 年期间意大利国家辅助生殖技术登记处收集的最新数据。卵母细胞冷冻保存的数据也与胚胎冷冻保存和相对新鲜卵母细胞 IVF 的结果进行了比较。
在 2009-2014 年期间，玻璃化冷冻保存卵母细胞的存活率、着床率和妊娠率明显高于慢速冷冻；然而，由于操作数量的不同，各中心的成功率仍存在很大差异。
由于玻璃化冷冻保存比慢速冷冻保存效果更好，因此现在已成为卵母细胞冷冻保存的首选方法，但仍需要更标准化的应用。与使用冷冻卵母细胞获得的胚胎相比，新鲜或冷冻胚胎的移植仍然显示出统计学上更好的性能。只有在少数冷冻保存经验丰富的中心，冷冻胚胎移植或冷冻卵母细胞获得胚胎的移植结果才具有可比性。
