Food Science and Technology, Oregon State University , Corvallis, Oregon 97330, United States.
Dietetics and Nutrition, Kansas University Medical Center , Kansas City, Kansas 66160, United States.
J Agric Food Chem. 2016 Oct 12;64(40):7492-7501. doi: 10.1021/acs.jafc.6b03401. Epub 2016 Sep 30.
Soy isoflavones exert beneficial health effects; however, their potential to ameliorate conditions associated with the metabolic syndrome (MetS) has not been studied in detail. In vitro and in vivo models were used to determine the effect of isoflavones on lipid metabolism, inflammation, and oxidative stress. In nude mice, consumption of Novasoy (NS) increased cholesterol and lipid metabolism gene expression, including Scd-1 (27.7-fold), Cyp4a14 (35.2-fold), and Cyp4a10 (9.5-fold), and reduced anti-inflammatory genes, including Cebpd (16.4-fold). A high-fat (HF) diet containing 0.4% (w/w) NS for 10 weeks significantly reduced percent weight gain (74.6 ± 2.5 vs 68.6 ± 3.5%) and hepatic lipid accumulation (20 ± 1.2 vs 27 ± 1.5%), compared to HF alone (p < 0.05) in C57BL/6J mice. NS also increased lipid oxidation and antioxidant gene expression while decreasing inflammatory cytokines. In vitro analysis in HepG2 cells revealed that genistein dose-dependently decreases oleic acid-induced lipid accumulation. Soy isoflavones may ameliorate symptoms associated with MetS via anti-inflammatory, antioxidant, and hypolipidemic modulation.
大豆异黄酮具有有益的健康作用；然而，其改善代谢综合征（MetS）相关病症的潜力尚未得到详细研究。本研究使用体外和体内模型来确定异黄酮对脂代谢、炎症和氧化应激的影响。在裸鼠中，食用 Novasoy（NS）增加了胆固醇和脂质代谢基因的表达，包括 Scd-1（27.7 倍）、Cyp4a14（35.2 倍）和 Cyp4a10（9.5 倍），并降低了抗炎基因，包括 Cebpd（16.4 倍）。在 C57BL/6J 小鼠中，10 周内用含有 0.4%（w/w）NS 的高脂肪（HF）饮食喂养可显著降低体重增加百分比（74.6 ± 2.5%比 68.6 ± 3.5%）和肝脏脂质积累（20 ± 1.2%比 27 ± 1.5%）（p < 0.05）。NS 还增加了脂质氧化和抗氧化基因的表达，同时减少了炎症细胞因子。在 HepG2 细胞中的体外分析表明，染料木黄酮可剂量依赖性地减少油酸诱导的脂质积累。大豆异黄酮可能通过抗炎、抗氧化和降血脂作用来改善 MetS 相关症状。
