Luo Ting, Goldfinger Tedd, Shay Neil
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.
Food Science and Technology, Oregon State University, Corvallis, OR, USA.
Curr Dev Nutr. 2020 Mar 13;4(4):nzaa033. doi: 10.1093/cdn/nzaa033. eCollection 2020 Apr.
Wine aged in oak barrels will incorporate polyphenols inherent in the staves, suggesting that wine stored in these wooden containers will introduce oak compounds into the human body after consumption.
The purpose of the present study is to test whether consumption of these oak compounds could favorably influence metabolism in mice fed an obesogenic diet.
C57BL/6 male mice ( = 8) were fed diets for 10 wk as follows: low-fat (LF), high-fat (HF), and HF containing 0.17% of oak tannin (HF+OT). A second 10-wk study was completed; mice were provided LF, HF, and HF diets supplemented with 7.0% of concentrates made from oaked wine (HF+OWC) or unoaked wine (HF+UWC). Physiological parameters were measured during the feeding trial and serum markers and hepatic gene expression measured from samples obtained at necropsy.
Intake of HF+OT significantly reduced body-weight gain (18.4 ± 1.2 g in HF vs. 13.2 ± 1.4 g in HF+OT, < 0.05). Serum resistin concentrations were lower in HF+OT mice compared with HF mice (301 ± 10.1 pg/mL in HF+OT vs. 374 ± 10.9 pg/mL in HF; < 0.05). Hepatic lipid accumulation and expression of glutathione--transferase-m2 () and NAD(P)H:quinone oxidoreductase () mRNAs were significantly decreased in HF+OT compared with HF mice ( < 0.05). When compared with HF-fed mice, intake of both OWC and UWC decreased body-weight gain ( < 0.05), with no significant impact on food consumption. Fasting glucose concentrations, serum insulin, and hepatic lipid accumulation were reduced in HF+OWC-fed mice compared with HF+UWC-fed mice ( < 0.05). Furthermore, hepatic glutathione--transferase-a1 () mRNA levels were significantly reduced in OWC-supplemented (0.25 ± 0.08) compared with UWC-supplemented (1.71 ± 0.24) mice ( < 0.05).
In this mouse model of metabolic disease, intake of OTs and a concentrate made from an oaked wine had a potent impact on alleviating HF-induced metabolic syndrome. Thus, intake of OTs, provided passively in oaked wine or as a dietary supplement, may act as an agent to attenuate the markers of metabolic syndrome.
在橡木桶中陈酿的葡萄酒会吸收桶板中固有的多酚，这表明储存在这些木制容器中的葡萄酒在饮用后会将橡木成分引入人体。
本研究旨在测试摄入这些橡木成分是否能对喂食致肥胖饮食的小鼠的新陈代谢产生有益影响。
将8只C57BL/6雄性小鼠喂养10周，饮食如下：低脂（LF）、高脂（HF）以及含0.17%橡木单宁的高脂饮食（HF+OT）。另一项为期10周的研究完成；为小鼠提供LF、HF，以及补充了7.0%橡木桶陈酿葡萄酒制成的浓缩物（HF+OWC）或未橡木桶陈酿葡萄酒制成的浓缩物（HF+UWC）的HF饮食。在喂养试验期间测量生理参数，并从尸检获得的样本中测量血清标志物和肝脏基因表达。
摄入HF+OT显著降低体重增加（HF组为18.4±1.2克，HF+OT组为13.2±1.4克，P<0.05）。与HF小鼠相比，HF+OT小鼠的血清抵抗素浓度较低（HF+OT组为301±10.1皮克/毫升，HF组为374±10.9皮克/毫升；P<0.05）。与HF小鼠相比，HF+OT组的肝脏脂质积累以及谷胱甘肽-S-转移酶-m2（GSTM2）和NAD（P）H：醌氧化还原酶（NQO1）mRNA的表达显著降低（P<0.05）。与喂食HF的小鼠相比，摄入OWC和UWC均降低了体重增加（P<0.05），对食物消耗没有显著影响。与喂食HF+UWC的小鼠相比，喂食HF+OWC的小鼠的空腹血糖浓度、血清胰岛素和肝脏脂质积累降低（P<0.05）。此外，与补充UWC（1.71±0.24）的小鼠相比，补充OWC（0.25±0.08）的小鼠肝脏谷胱甘肽-S-转移酶-a1（GSTA1）mRNA水平显著降低（P<0.05）。
在这个代谢疾病小鼠模型中，摄入橡木单宁和橡木桶陈酿葡萄酒制成的浓缩物对缓解HF诱导的代谢综合征有显著影响。因此，在橡木桶陈酿葡萄酒中被动提供或作为膳食补充剂摄入橡木单宁，可能作为一种减轻代谢综合征标志物的物质。
