Zhao J Y, Mu Y J, Zhang B F, Xia S L, Su J, Huang X Y, Xu B L
Pathogenic Microbiology Laboratory of Institute for Infectious Disease Control and Prevention, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2016 Sep 10;37(9):1253-1256. doi: 10.3760/cma.j.issn.0254-6450.2016.09.014.
To analyze the serotypes and antibiotic resistance phenotypes of non-typhi strains in Henan province from 2011 to 2015. The stool samples were collected from diarrhea patients, and enriched with SBG enrichment broth and the pathogen isolation was conducted with CHROMAgar selective culture medium at 37 ℃ for 18-24 hours using KIA/MIU biochemical action and API20E biochemical system slab to identify strains. The serotypes of all the positive strains were detected with SSI typing sera. According to K-B drug susceptibility testing method published by USA clinical and Laboratory Standards Institute (CLSI), the antibiotics resistant phenotype of the positive strains were analyzed. A total of 1 351 strains of non-typhi were isolated, in which 811 were from males, 540 were from females. The ratio of men to women was 1.5∶1. Children and young adults were mainly affected. The pathogen isolation was mainly in May-October during a year. The 1 351 strains of non-typhi were divided into 58 serotypes. and ranked 1-7. The drug-resistance rate of the 1 351 strains was 46.1% to synthetic broad-spectrum penicillin ampicillin (AMP), 19.5% and 21.2% to the three generation cephalosporin ceftazidime (CAZ) and cefotaxime (CTX), 8.8% to the four generation cephalosporins cefepime (FEP), 58.7% to the one generation of quinolones nalidixic acid (NAL), 14.7% to the three generation fluoroquinolone ciprofloxacin (CIP) and norfloxacin (NOR), 25.0% and 35.6% to aminoglycoside antibiotic gentamicin (GEN) and streptomycin (STR), 35.4% to amphenicols (CHL), 31.7% to sulfonamide trimethoprim (TMP) and 37.8% to tetracycline (TET). Totally 879 strains were multidrug resistant (65.1%): 350 strains were resistant to 3-4 kinds of antibiotics (25.9%), 309 strains were resistant to 5-7 kinds of antibiotics (22.9%), 174 strains were resistant to 8-10 kinds of antibiotics (12.9%) and 48 strains were resistant to 11-12 kinds of antibiotics (3.6%). The serotypes of non-typhi strains isolated from Henan province varied, some strains were resistant to 12 kinds of antibiotics commonly used in clinical treatment and the multidrug resistance has become serious.
分析2011年至2015年河南省非伤寒 菌株的血清型及抗生素耐药表型。采集腹泻患者粪便样本，用SBG增菌肉汤增菌，采用CHROMAgar选择性培养基在37℃培养18 - 24小时进行病原菌分离，并用KIA/MIU生化反应及API20E生化系统鉴定菌株。用SSI分型血清检测所有阳性菌株的血清型。根据美国临床和实验室标准协会（CLSI）公布的K - B药敏试验方法，分析阳性菌株的抗生素耐药表型。共分离出1351株非伤寒 菌株，其中男性811株，女性540株。男女比例为1.5∶1。主要感染儿童和青壮年。病原菌分离主要集中在一年中的5 - 10月。1351株非伤寒 菌株分为58个血清型， 排前1 - 7位。1351株菌株对合成广谱青霉素氨苄西林（AMP）的耐药率为46.1%，对第三代头孢菌素头孢他啶（CAZ）和头孢噻肟（CTX）的耐药率分别为19.5%和21.2%，对第四代头孢菌素头孢吡肟（FEP）的耐药率为8.8%，对第一代喹诺酮萘啶酸（NAL）的耐药率为58.7%，对第三代氟喹诺酮环丙沙星（CIP）和诺氟沙星（NOR）的耐药率分别为14.7%，对氨基糖苷类抗生素庆大霉素（GEN）和链霉素（STR）的耐药率分别为25.0%和35.6%，对氯霉素（CHL）的耐药率为35.4%，对磺胺甲恶唑（TMP）的耐药率为31.7%，对四环素（TET）的耐药率为37.8%。共有879株多重耐药（65.1%）：350株对3 - 4种抗生素耐药（25.9%），309株对5 - 7种抗生素耐药（22.9%），174株对8 - 10种抗生素耐药（
