双子座：小鼠颈脊髓完全离断后的初步行为学结果。
GEMINI: Initial behavioral results after full severance of the cervical spinal cord in mice.
作者信息
Kim C-Yoon, Oh Hanseul, Hwang In-Kyu, Hong Ki-Sung
机构信息
Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Korea; Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
出版信息
Surg Neurol Int. 2016 Sep 13;7(Suppl 24):S629-31. doi: 10.4103/2152-7806.190474. eCollection 2016.
BACKGROUND
The GEMINI spinal cord fusion protocol has been developed to achieve a successful cephalosomatic anastomosis. Here, we report the preliminary data on the use of a fusogen [polyethylene glycol (PEG)] after full cervical cord transection in mice to facilitate the fusion of both ends of a sharply transected spinal cord.
METHODS
Cervical laminectomy and a complete, visually confirmed cervical cord (C 5) transection was performed on female albino mice (n = 16). In Group 1 (n = 8), a fusogen, (PEG) was used to bridge the gap between the cut ends of the spinal cord. Group 2 received the same spinal cord transection but was treated with saline. Outcome was assessed daily using a standard scale (modified 22-point Basso-Beattie-Bresnahan scale) and filmed on camera.
RESULTS
The PEG group (group 1) showed partial restoration of motor function after 4 weeks of observation; group 2 (placebo) did not recover any useful motor activity.
CONCLUSION
In this preliminary experiment, PEG, but not saline, promoted partial motor recovery in mice submitted to full cervical transection.
背景
已开发出双子座脊髓融合方案以实现成功的头体吻合。在此，我们报告在小鼠全颈髓横断后使用融合剂[聚乙二醇（PEG）]促进锐性横断脊髓两端融合的初步数据。
方法
对雌性白化小鼠（n = 16）进行颈椎板切除术，并进行完全的、经视觉确认的颈髓（C5）横断。在第1组（n = 8）中，使用融合剂（PEG）桥接脊髓切断端之间的间隙。第2组接受相同的脊髓横断，但用盐水治疗。每天使用标准量表（改良的22分Basso-Beattie-Bresnahan量表）评估结果，并拍摄视频。
结果
观察4周后，PEG组（第1组）显示运动功能部分恢复；第2组（安慰剂组）未恢复任何有用的运动活动。
结论
在这项初步实验中，PEG而非盐水促进了全颈髓横断小鼠的部分运动恢复。
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