Qu Zhipeng, Zhang Hai, Huang Moli, Shi Guangsen, Liu Zhiwei, Xie Pancheng, Li Hui, Wang Wei, Xu Guoqiang, Zhang Yang, Yang Ling, Huang Guocun, Takahashi Joseph S, Zhang Weiping J, Xu Ying
MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, China.
Department of Pathophysiology, Second Military Medical University, Shanghai, China.
Elife. 2016 Sep 22;5:e17171. doi: 10.7554/eLife.17171.
Many animals display morning and evening bimodal activities in the day/night cycle. However, little is known regarding the potential components involved in the regulation of bimodal behavioral rhythms in mammals. Here, we identified that the zinc finger protein gene plays a crucial role in the regulation of bimodal activities in mice. Depletion of in nerve system resulted in the loss of early evening activity, but the increase of morning activity. We found that -deficient mice exhibited a pronounced decrease in the expression of and resembled phenotypes of and -knockout mice. Injection of adeno-associated virus-double-floxed in suprachiasmatic nucleus could partly restore evening activity in (NS-ZB20KO) mice. Furthermore, loss of in loci, but intact in the suprachiasmatic nucleus, was not responsible for the unimodal activity of NS-ZB20KO mice. Our study provides evidence that ZBTB20-mediated PROKR2 signaling is critical for the evening behavioral rhythms.
许多动物在昼夜周期中表现出早晚双峰活动。然而，关于哺乳动物双峰行为节律调节中潜在的参与成分，我们知之甚少。在此，我们确定锌指蛋白基因在小鼠双峰活动的调节中起关键作用。神经系统中该基因的缺失导致傍晚早期活动丧失，但早晨活动增加。我们发现该基因缺陷小鼠中某一基因的表达明显降低，且类似于另一基因敲除小鼠的表型。在视交叉上核注射腺相关病毒双loxP侧翼序列的该基因可部分恢复（NS-ZB20KO）小鼠的傍晚活动。此外，在某一基因位点缺失但视交叉上核完整，并非NS-ZB20KO小鼠单峰活动的原因。我们的研究提供了证据表明ZBTB20介导的PROKR2信号传导对傍晚行为节律至关重要。
