Nicolaus Mackenzie L, Bergdall Valerie K, Davis Ian C, Hickman-Davis Judy M
Office of Research, University Laboratory Animal Resources, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
J Am Assoc Lab Anim Sci. 2016;55(5):525-33.
Food availability, temperature, humidity, strain, and caging type all affect water consumption by mice. Measurement of transepidermal water loss (TEWL) is a new technique for the quantification of water turnover in mice. To understand water turnover in common strains of adult mice, male and female SCID, SKH, C57BL/6, and FVB mice were housed in same-sex groups of 5 animals in static cages or IVC. Body weight, TEWL, urine osmolality, and water consumption of mice and intracage temperature and humidity were measured every 48 h for comparison. Static cages were monitored for 7 d and IVC for 14 d before cage change. Female SCID, FVB, and C57 mice drank less water than did their male counterparts. Male and female SCID, SKH, and FVB mice in IVC drank less water and had higher urine osmolality than did those in static cages. In SCID and SKH mice, TEWL paralleled water consumption. C57 mice in static cages drank less water, had lower urine osmolality, and had less TEWL than did those in IVC. Temperature and humidity within the cage was higher than the macroenvironmental levels for all housing conditions, mouse strains, and sexes. Temperatures within IVC ranged from 76.6 to 81.4 °F compared with 69±0.4 °F in the room. Humidity within IVC ranged from 68% to 79% compared with 27.o%±2.7% within the room. These data demonstrate that mouse strain and housing conditions significantly influence water balance and indicate that macroenvironmental measurements do not always reflect the intracage environment.
食物供应、温度、湿度、品系以及饲养笼类型都会影响小鼠的水消耗。经表皮水分流失（TEWL）测量是一种用于量化小鼠水分周转率的新技术。为了解成年小鼠常见品系的水分周转率，将雄性和雌性SCID、SKH、C57BL/6和FVB小鼠以每组5只同性别的方式饲养在静态笼或独立通风笼盒（IVC）中。每48小时测量小鼠的体重、TEWL、尿渗透压、水消耗量以及笼内温度和湿度以作比较。在更换笼子前，对静态笼监测7天，对IVC监测14天。雌性SCID、FVB和C57小鼠比雄性小鼠饮水少。IVC中的雄性和雌性SCID、SKH和FVB小鼠比静态笼中的小鼠饮水少且尿渗透压更高。在SCID和SKH小鼠中，TEWL与水消耗量平行。静态笼中的C57小鼠比IVC中的C57小鼠饮水少、尿渗透压更低且TEWL更少。对于所有饲养条件、小鼠品系和性别，笼内温度和湿度均高于宏观环境水平。IVC内的温度范围为76.6至81.4°F，而室内温度为69±0.4°F。IVC内的湿度范围为68%至79%，而室内湿度为27.0%±2.7%。这些数据表明小鼠品系和饲养条件显著影响水平衡，并表明宏观环境测量并不总是反映笼内环境。
