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咖啡因正向调节H460细胞中铁蛋白重链的表达：对细胞增殖的影响。

Caffeine Positively Modulates Ferritin Heavy Chain Expression in H460 Cells: Effects on Cell Proliferation.

作者信息

Zolea Fabiana, Biamonte Flavia, Battaglia Anna Martina, Faniello Maria Concetta, Cuda Giovanni, Costanzo Francesco

机构信息

Research Center of Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Salvatore Venuta Campus, Viale Europa, 88100, Catanzaro, Italy.

出版信息

PLoS One. 2016 Sep 22;11(9):e0163078. doi: 10.1371/journal.pone.0163078. eCollection 2016.

DOI:10.1371/journal.pone.0163078
PMID:27657916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5033359/
Abstract

Both the methylxanthine caffeine and the heavy subunit of ferritin molecule (FHC) are able to control the proliferation rate of several cancer cell lines. While caffeine acts exclusively as a negative modulator of cell proliferation, FHC might reduce or enhance cell viability depending upon the different cell type. In this work we have demonstrated that physiological concentrations of caffeine reduce the proliferation rate of H460 cells: along with the modulation of p53, pAKT and Cyclin D1, caffeine also determines a significant FHC up-regulation through the activation of its transcriptional efficiency. FHC plays a central role in the molecular pathways modulated by caffeine, ending in a reduced cell growth, since its specific silencing by siRNA almost completely abolishes caffeine effects on H460 cell proliferation. These results allow the inclusion of ferritin heavy subunits among the multiple molecular targets of caffeine and open the way for studying the relationship between caffeine and intracellular iron metabolism.

摘要

甲基黄嘌呤咖啡因和铁蛋白分子的重链亚基（FHC）都能够控制多种癌细胞系的增殖速率。虽然咖啡因仅作为细胞增殖的负调节剂发挥作用，但FHC可能会根据不同的细胞类型降低或增强细胞活力。在这项研究中，我们证明生理浓度的咖啡因会降低H460细胞的增殖速率：除了调节p53、pAKT和细胞周期蛋白D1外，咖啡因还通过激活其转录效率导致FHC显著上调。FHC在咖啡因调节的分子途径中起核心作用，最终导致细胞生长减少，因为通过小干扰RNA对其进行特异性沉默几乎完全消除了咖啡因对H460细胞增殖的影响。这些结果使得铁蛋白重链亚基被纳入咖啡因的多个分子靶点之中，并为研究咖啡因与细胞内铁代谢之间的关系开辟了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/e36879e20731/pone.0163078.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/f71b97f8372c/pone.0163078.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/39f0c5515ab3/pone.0163078.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/9bd360651aa1/pone.0163078.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/85834d5f2398/pone.0163078.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/e36879e20731/pone.0163078.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/f71b97f8372c/pone.0163078.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/aa2359d58ae4/pone.0163078.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/39f0c5515ab3/pone.0163078.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/9bd360651aa1/pone.0163078.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/85834d5f2398/pone.0163078.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f5e/5033359/e36879e20731/pone.0163078.g006.jpg

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