DiCicco-Bloom E, Black I B
Department of Neurology, Cornell University Medical College, New York, NY 10021.
Brain Res. 1989 Jul 10;491(2):403-6. doi: 10.1016/0006-8993(89)90080-2.
Although neuroblast generation is highly reproducible temporospatially, the underlying regulation is undefined. Employing a fully defined sympathetic neuroblast culture system, we previously found that insulin growth factors regulated the mitotic cycle. We now report that depolarizing stimuli, including elevated K+ and veratridine, also increased the proportion of mitotic neuroblasts in vitro. Moreover, Na+ channel blockade prevented effects of depolarization, but not that of insulin-like growth factor I, suggesting that these influences stimulate mitosis by different membrane transduction mechanisms.
尽管神经母细胞的生成在时空上具有高度的可重复性，但其潜在的调控机制尚不清楚。利用一个完全明确的交感神经母细胞培养系统，我们之前发现胰岛素生长因子调节有丝分裂周期。我们现在报告，包括高K⁺和藜芦碱在内的去极化刺激，在体外也增加了有丝分裂神经母细胞的比例。此外，Na⁺通道阻断可阻止去极化的作用，但不能阻止胰岛素样生长因子I的作用，这表明这些影响是通过不同的膜转导机制刺激有丝分裂的。
