一种用于制作膜片钳微吸管的改良型水平毛细管拉制仪。

A modified horizontal capillary puller for fabrication of patch-clamp pipettes.

作者信息

Mealing G A, Schwartz J L

机构信息

Division of Biological Sciences, National Research Council, Ottawa, Ontario, Canada.

出版信息

Brain Res Bull. 1989 May;22(5):913-5. doi: 10.1016/0361-9230(89)90036-1.

DOI:10.1016/0361-9230(89)90036-1

PMID:2765950

Abstract

A simple low-cost modification to a conventional horizontal two-stage micropipette puller is described. It allows the production of pipettes suitable for patch-clamp studies. It requires that the hard-pull stage of the fabrication process be disabled and two heating cycles, each with a different heating filament temperature, be employed. The modification is described specifically for an Industrial Science Associates, Inc. M-1 micropipette puller. However, in principle it should be applicable to any horizontal two-stage puller using a solenoid to generate the pull force.

摘要

本文描述了一种对传统卧式两级微量移液器拉制器的简单低成本改进方法。它能够生产适用于膜片钳研究的移液器。这需要在制造过程中禁用强力拉制阶段，并采用两个加热周期，每个周期的加热丝温度不同。文中具体针对工业科学协会公司的M-1型微量移液器拉制器描述了这种改进方法。然而，原则上它应适用于任何使用螺线管产生拉力的卧式两级拉制器。