Chu Ci, Chang Howard Y
Center for Personal Dynamic Regulomes, Stanford University, 269 Campus Drive, Stanford, CA, 94305, USA.
Methods Mol Biol. 2016;1480:115-23. doi: 10.1007/978-1-4939-6380-5_10.
ChIRP is a novel and easy-to-use technique for studying long noncoding RNA (lncRNA)-chromatin interactions. RNA and chromatin are cross-linked in vivo using formaldehyde or glutaraldehyde, and purified using biotinylated antisense oligonucleotides that hybridize to the target RNA. Co-precipitated DNA is then purified and analyzed by quantitative PCR (qPCR) or high-throughput sequencing.
ChIRP是一种用于研究长链非编码RNA（lncRNA）与染色质相互作用的新颖且易于使用的技术。使用甲醛或戊二醛在体内使RNA与染色质交联，然后使用与靶RNA杂交的生物素化反义寡核苷酸进行纯化。接着对共沉淀的DNA进行纯化，并通过定量PCR（qPCR）或高通量测序进行分析。
