Morey Lluis, Di Croce Luciano
Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003, Barcelona, Spain.
Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 1475 N.W. 12th Avenue, Miami, FL, 33136, USA.
Methods Mol Biol. 2016;1480:153-65. doi: 10.1007/978-1-4939-6380-5_14.
PRC1 complexes contain four core subunits: Pcgf, Phc, Ring1, and Cbx proteins. Interestingly, mammalian genomes have several paralogues for each subunit, which are differentially expressed depending on the cell type, differentiation program, and cellular stimuli. Therefore, identification and characterization of the specific architecture of different PRC1 complexes during cellular differentiation are essential to better understand the function and recruitment mechanism of PRC1 complexes. In this chapter we describe several methods to study Polycomb architecture, and identification of novel interactors in both pluripotent and differentiating mouse embryonic stem cells.
PRC1复合物包含四个核心亚基：Pcgf、Phc、Ring1和Cbx蛋白。有趣的是，哺乳动物基因组中每个亚基都有几个旁系同源物，它们根据细胞类型、分化程序和细胞刺激而差异表达。因此，在细胞分化过程中鉴定和表征不同PRC1复合物的特定结构对于更好地理解PRC1复合物的功能和招募机制至关重要。在本章中，我们描述了几种研究多梳结构的方法，以及在多能和分化的小鼠胚胎干细胞中鉴定新型相互作用分子的方法。
