追踪果蝇活体胚胎中多梳蛋白体的运动

Following the Motion of Polycomb Bodies in Living Drosophila Embryos.

作者信息

Cheutin Thierry, Cavalli Giacomo

机构信息

Institut de Génétique Humaine, CNRS UPR 1142, 141, rue de la Cardonille, 34396, Montpellier Cedex 5, France.

出版信息

Methods Mol Biol. 2016;1480:283-8. doi: 10.1007/978-1-4939-6380-5_24.

DOI:10.1007/978-1-4939-6380-5_24

PMID:27659993

Abstract

During the last two decades, observation of cell nuclei in live microscopy evidences motion of nuclear compartments. Drosophila embryos constitute a good model to study nuclear dynamic during cell differentiation because they can easily be observed in live microscopy. Inside the cell nucleus, Polycomb group proteins accumulate in foci named Pc bodies. Here, we describe a method to visualize and analyze the motion of these nuclear compartments inside cell nuclei of Drosophila embryos.

摘要

在过去二十年中，通过活细胞显微镜观察细胞核发现了核区室的运动。果蝇胚胎是研究细胞分化过程中核动态的良好模型，因为可以很容易地通过活细胞显微镜对其进行观察。在细胞核内，多梳蛋白家族蛋白在名为多梳小体（Pc bodies）的位点积累。在此，我们描述了一种可视化和分析果蝇胚胎细胞核内这些核区室运动的方法。