Lee Jae-Young, Park Sunghyun, Kim Kwang-Soo, Ko Jeong-Jae, Lee Soohong, Kim Keun Pil, Park Kyung-Soon
Dept. of Biomedical Science, College of Life Science, CHA University, Seoul 06135, Korea.
Dept. of Life Science, Chung-Ang University, Seoul 06975, Korea.
Dev Reprod. 2016 Jun;20(2):171-7. doi: 10.12717/DR.2016.20.2.171.
Sprouty (Spry) genes encode inhibitors of the receptor tyrosine kinase signaling cascade, which plays important roles in stem cells. However, the role of Spry4 in the stemness of embryonic stem cells has not been fully elucidated. Here, we used mouse embryonic stem cells (mESCs) as a model system to investigate the role of Spry4 in the stem cells. Suppression of Spry4 expression results in the decreases of cell proliferation, EB formation and stemness marker expression, suggesting that Spry4 activity is associated with stemness of mESCs. Teratoma assay showed that the cartilage maturation was facilitated in Spry4 knocked down mESCs. Our results suggest that Spry4 is an important regulator of the stemness and differentiation of mESCs.
Sprouty（Spry）基因编码受体酪氨酸激酶信号级联的抑制剂，该信号级联在干细胞中发挥重要作用。然而，Spry4在胚胎干细胞干性中的作用尚未完全阐明。在这里，我们使用小鼠胚胎干细胞（mESCs）作为模型系统来研究Spry4在干细胞中的作用。Spry4表达的抑制导致细胞增殖、胚体形成和干性标志物表达的降低，表明Spry4活性与mESCs的干性相关。畸胎瘤实验表明，在Spry4敲低的mESCs中软骨成熟得到促进。我们的结果表明，Spry4是mESCs干性和分化的重要调节因子。
