Kim Gap-Don, Seo Jin-Kyu, Yum Hyeon-Woong, Jeong Jin-Yeon, Yang Han-Sul
Department of Animal Sciences, University of Illinois at Urbana-Champaign, 1503 S Maryland Drive, Urbana, IL 61801, USA; Institute of Agriculture and Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea.
Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea.
Food Chem. 2017 Feb 15;217:163-170. doi: 10.1016/j.foodchem.2016.08.100. Epub 2016 Aug 27.
The purpose of this study was to find discrimination markers for four major meat species such as beef, pork, chicken and duck. Myofibrillar and sarcoplasmic proteins isolated from each meat type were analyzed by one-dimensional gel electrophoresis and some proteins were identified through LC-MS/MS analysis. We confirmed that troponin I (TnI), enolase 3, l-lactate dehydrogenase (LDH) and triose-phosphate isomerase (TPI) could be useful markers for discrimination of mammals from poultry due to their different electrophoretic mobility. Tropomyosin 1 and carbonic anhydrase 3 were observed as muscle fiber type-related proteins and these could also be markers to distinguish mammals from poultry. Species-specific peptides identified by LC-MS/MS spectra allow the identification of each species regardless of the same protein. Therefore, it is easy to discriminate between mammals and poultry by comparing the electrophoretic mobility of TnI, enolase 3, LDH, TPI and CA3, and each species could be identified through LC-MS/MS analysis.
本研究的目的是寻找牛肉、猪肉、鸡肉和鸭肉这四种主要肉类的鉴别标志物。从每种肉类中分离出的肌原纤维蛋白和肌浆蛋白通过一维凝胶电泳进行分析，并通过液相色谱-串联质谱（LC-MS/MS）分析鉴定了一些蛋白质。我们证实，肌钙蛋白I（TnI）、烯醇化酶3、L-乳酸脱氢酶（LDH）和磷酸丙糖异构酶（TPI）由于其不同的电泳迁移率，可作为区分哺乳动物和禽类的有用标志物。原肌球蛋白1和碳酸酐酶3被观察为与肌纤维类型相关的蛋白质，它们也可作为区分哺乳动物和禽类的标志物。通过LC-MS/MS光谱鉴定的物种特异性肽允许识别每个物种，而不管蛋白质是否相同。因此，通过比较TnI、烯醇化酶3、LDH、TPI和CA3的电泳迁移率，很容易区分哺乳动物和禽类，并且可以通过LC-MS/MS分析鉴定每个物种。
