一种针对兔孕激素受体的多克隆抗血清可识别人类受体：在兔和人类子宫中的免疫组织化学定位
A polyclonal antiserum against the rabbit progesterone receptor recognizes the human receptor: immunohistochemical localization in rabbit and human uterus.
作者信息
Zaino R J, Feil P D, Clarke C L, Mortel R, Satyaswaroop P G
机构信息
Department of Pathology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
出版信息
Cell Biochem Funct. 1989 Apr;7(2):147-52. doi: 10.1002/cbf.290070211.
A polyclonal antiserum, raised in guinea pigs immunized with the 116,000 Mr rabbit uterine progesterone receptor (PR), was used to demonstrate immunoreactive PR in frozen fixed sections of rabbit and human uterus. In both species, PR localization was exclusively nuclear. For the rabbit uterus, staining intensity was greatest in the myometrium, followed by endometrial stroma, glands, and luminal epithelium. In premenopausal human endometrium and myometrium there was intense staining of nuclei from proliferative phase glands and myometrium. In the secretory phase the glands failed to stain, yet immunostaining persisted in the myometrium.
用116,000道尔顿的兔子宫孕酮受体（PR）免疫豚鼠制备的多克隆抗血清，用于在兔和人子宫的冷冻固定切片中检测免疫反应性PR。在这两个物种中，PR均仅定位于细胞核。对于兔子宫，染色强度在肌层中最大，其次是子宫内膜基质、腺体和腔上皮。在绝经前的人子宫内膜和肌层中，增殖期腺体和肌层的细胞核有强烈染色。在分泌期，腺体未染色，但肌层仍有免疫染色。
Zotero 插件