Rasio E A, Bendayan M, Goresky C A, Alexander J S, Shepro D
Department of Medicine, Notre-Dame Hospital, Montréal, QC, Canada.
Circ Res. 1989 Sep;65(3):591-9. doi: 10.1161/01.res.65.3.591.
The effects of 10(-6) M phalloidin on reperfusion-injured blood capillary structure and permeability were studied in the countercurrent perfused rete mirabile of the eel swim bladder. In the normal rete, the addition of phalloidin to the perfusion medium did not induce morphological or functional changes. When flow was arrested for 30 minutes, during which time the capillaries were exposed to inhibitors of ATP generation, and flow was then resumed with an oxygenated medium, cell membrane blebs and vacuolization, mitochondrial swelling, pericyte shrinkage, and interstitial space edema were observed. The permeability coefficients for labeled albumin, sucrose, and sodium increased to three to four times baseline values, whereas the permeability to water was not significantly modified. When the same protocol was repeated with phalloidin present in the medium throughout the experiment, the structural integrity of the endothelial cells was completely preserved and pericyte shrinkage was abolished, but interstitial space edema still occurred. The permeability to albumin, sucrose, and sodium increased only to 1.5 times baseline values, a significantly decreased increment in comparison with the experiments performed without phalloidin. We concluded that although phalloidin does not improve the capillary barrier of the normal rete, it provides protection against the structural and functional damage induced by hypoxia and reperfusion.
在鳗鱼鳔逆流灌注的神奇网中，研究了10⁻⁶ M鬼笔环肽对再灌注损伤的毛细血管结构和通透性的影响。在正常的神奇网中，向灌注培养基中添加鬼笔环肽不会引起形态或功能变化。当血流停止30分钟，在此期间毛细血管暴露于ATP生成抑制剂，然后用含氧培养基恢复血流时，观察到细胞膜泡化和空泡化、线粒体肿胀、周细胞收缩和间质水肿。标记白蛋白、蔗糖和钠的通透性系数增加到基线值的三到四倍，而对水的通透性没有明显改变。当在整个实验过程中培养基中都存在鬼笔环肽重复相同方案时，内皮细胞的结构完整性得到完全保留，周细胞收缩被消除，但间质水肿仍然发生。白蛋白、蔗糖和钠的通透性仅增加到基线值的1.5倍，与没有鬼笔环肽的实验相比，增加幅度明显降低。我们得出结论，虽然鬼笔环肽不能改善正常神奇网的毛细血管屏障，但它能保护免受缺氧和再灌注诱导的结构和功能损伤。
