Rama Estefanía C, Costa-García Agustín, Fernández-Abedul M Teresa
Departamento de Química Física y Analítica, Universidad de Oviedo , 33006, Oviedo, Spain.
Anal Chem. 2016 Oct 18;88(20):9958-9963. doi: 10.1021/acs.analchem.6b01609. Epub 2016 Oct 3.
This work describes the use of mass-fabricated stainless-steel pins as new low-cost electrodes for a flow injection analysis (FIA) system with electrochemical detection. The pins serving as electrodes are directly punched in the tubing where solutions flow, being one of the simplest flow cells for FIA. This cell consists of a carbon ink coated pin as working electrode and two bare pins as counter and reference electrodes. The pins are able to perform at least 300 measurements. Moreover, they can be easily replaced showing good repeatability and reproducibility (RSD lower than 6% in all the cases). As a proof-of-concept, the feasibility of the system to determine glucose was evaluated by an enzymatic assay using glucose oxidase, horseradish peroxidase, and ferrocyanide as electron-transfer mediator. The application of this system to real food samples has shown accurate results.
这项工作描述了使用大规模制造的不锈钢针作为新型低成本电极，用于带有电化学检测的流动注射分析（FIA）系统。用作电极的针直接冲压在溶液流动的管道中，是FIA最简单的流通池之一。该流通池由涂有碳墨的针作为工作电极，两个裸针作为对电极和参比电极组成。这些针能够进行至少300次测量。此外，它们可以很容易地更换，显示出良好的重复性和再现性（在所有情况下相对标准偏差均低于6%）。作为概念验证，使用葡萄糖氧化酶、辣根过氧化物酶和亚铁氰化物作为电子转移介质，通过酶促测定法评估了该系统测定葡萄糖的可行性。该系统在实际食品样品中的应用已显示出准确的结果。
