Chen C Z, Sutherland J C
Biology Department, Brookhaven National Laboratory, Upton, NY 11973.
Electrophoresis. 1989 May-Jun;10(5-6):318-26. doi: 10.1002/elps.1150100509.
We describe a method based on gel electrophoresis for the quantitation of strand breaks in DNA and demonstrate its application to the measurement of single- and double-strand breaks formed by gamma-rays for DNA irradiated in vitro. For single-strand breaks, our data span the dose range from 0.1 to 1 Gy, while for double-strand breaks doses were from 3 to 15 Gy. In agreement with results obtained using other techniques, we find that the dose response function for single-strand breaks is linear while the dose response function for double-strand breaks is curved, indicating that it is the sum of both linear and quadratic components. We discuss factors that determine the sensitivity of the method and indicate approaches to make possible the quantitation of strand breaks in the DNA of cells irradiated with sublethal doses.
我们描述了一种基于凝胶电泳的方法，用于定量DNA中的链断裂，并展示了其在测量体外照射的DNA中由γ射线形成的单链和双链断裂方面的应用。对于单链断裂，我们的数据涵盖了0.1至1 Gy的剂量范围，而对于双链断裂，剂量范围为3至15 Gy。与使用其他技术获得的结果一致，我们发现单链断裂的剂量响应函数是线性的，而双链断裂的剂量响应函数是曲线的，这表明它是线性和二次成分的总和。我们讨论了决定该方法灵敏度的因素，并指出了实现对亚致死剂量照射细胞的DNA中的链断裂进行定量的方法。
