Solomon Benjamin D, Hsieh Chyi-Song
Division of Rheumatology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Division of Rheumatology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110
J Immunol. 2016 Nov 1;197(9):3512-3519. doi: 10.4049/jimmunol.1601217. Epub 2016 Sep 26.
Foxp3retinoic acid-related orphan receptor (ROR)γt T cells have recently been characterized as an immunoregulatory population highly enriched in the colon lamina propria. However, their developmental origin and relationship to RORγt regulatory T and Th17 cells remain unclear. In this study, we use a fixed TCRβ system to show that the TCR repertoire of the Foxp3RORγt population is mostly distinct compared with other colonic T cell subsets. However, of these TCRs, a fraction is also found in the Th17 subset, suggesting that TCR repertoire overlap may contribute to the reported ability of Foxp3RORγt cells to regulate Th17 immunity. Naive transgenic T cells expressing a Foxp3RORγt-restricted TCR first acquire a Foxp3RORγt phenotype before coexpressing RORγt, suggesting that Foxp3RORγt cell development can occur via an RORγt regulatory T cell intermediate.
叉头框蛋白3（Foxp3）维甲酸相关孤儿受体（ROR）γt T细胞最近被鉴定为在结肠固有层中高度富集的免疫调节细胞群体。然而，它们的发育起源以及与RORγt调节性T细胞和Th17细胞的关系仍不清楚。在本研究中，我们使用固定的TCRβ系统来表明，与其他结肠T细胞亚群相比，Foxp3RORγt细胞群体的TCR库大多是不同的。然而，在这些TCR中，有一部分也存在于Th17亚群中，这表明TCR库的重叠可能有助于Foxp3RORγt细胞调节Th17免疫的报道能力。表达受Foxp3RORγt限制的TCR的幼稚转基因T细胞在共表达RORγt之前首先获得Foxp3RORγt表型，这表明Foxp3RORγt细胞的发育可以通过RORγt调节性T细胞中间体发生。
