绿脓菌素作为抗酪氨酸酶和抗体癣药物：一项新的治疗研究。
Pyocyanin as anti-tyrosinase and anti tinea corporis: A novel treatment study.
作者信息
El-Zawawy Nessma A, Ali Sameh S
机构信息
Botany Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt.
Botany Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt; Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
出版信息
Microb Pathog. 2016 Nov;100:213-220. doi: 10.1016/j.micpath.2016.09.013. Epub 2016 Sep 23.
OBJECTIVE
The aim of this study was to evaluate the efficiency of pyocyanin pigment as a novel compound active against tyrosinase with its depigmentation efficiency for combating Trichophyton rubrum which could be a major causative agent of tinea corporis.
METHODS
Fifty swabs of fungal tinea corporis infections were collected and identified. Five MDRPA isolates were tested for their levels of pyocyanin production. The purified extracted pyocyanin was characterized by UV spectrum and FT-IR analysis. Pyocyanin activity against tyrosinase was determined by dopachrome micro-plate. In addition, the antidermatophytic activity of pyocyanin against T. rubrum was detected by radial growth technique. In vivo novel trial was conducted to evaluate the efficiency and safety of pyocyanin as an alternative natural therapeutic compound against T. rubrum causing tinea corporis.
RESULTS
Purified pyocyanin showed highly significant inhibitory activity against tyrosinase and T. rubrum. In vivo topical treatments with pyocyanin ointment revealed the efficiency of pyocyanin (MIC 2000 μg/ml) to cure tinea corporis compared to fluconazole, which showed a partial curing at a higher concentration (MIC 3500 μg/ml) after two weeks of treatment. In addition, the results revealed complete healing and disappear of hyperpigmentation by testing the safety of pyocyanin ointment and its histopathological efficiency in the skin treatment without any significant toxic effect.
CONCLUSION
Pyocyanin pigment could be a promising anti-tyrosinase and a new active compound against T. rubrum, which could be a major causative agent of tinea corporis. In fact, if pyocyanin secondary metabolite is going to be used in practical medication, it will support the continuous demand of novel antimycotic natural agents against troublesome fungal infections.
目的
本研究旨在评估绿脓菌素作为一种新型抗酪氨酸酶化合物的效率及其对红色毛癣菌的脱色效率，红色毛癣菌可能是体癣的主要病原体。
方法
收集并鉴定了50份体癣真菌感染拭子。测试了5株多重耐药铜绿假单胞菌分离株的绿脓菌素产生水平。通过紫外光谱和傅里叶变换红外光谱分析对纯化提取的绿脓菌素进行表征。采用多巴色素微孔板法测定绿脓菌素对酪氨酸酶的活性。此外，采用径向生长技术检测绿脓菌素对红色毛癣菌的抗皮肤癣菌活性。进行体内新试验以评估绿脓菌素作为抗红色毛癣菌引起体癣的替代天然治疗化合物的效率和安全性。
结果
纯化的绿脓菌素对酪氨酸酶和红色毛癣菌显示出高度显著的抑制活性。与氟康唑相比，用绿脓菌素软膏进行的体内局部治疗显示绿脓菌素（MIC 2000μg/ml）治愈体癣的效率，氟康唑在治疗两周后在较高浓度（MIC 3500μg/ml）下显示部分治愈。此外，通过测试绿脓菌素软膏的安全性及其在皮肤治疗中的组织病理学效率，结果显示色素沉着过度完全愈合并消失，且无任何明显毒性作用。
结论
绿脓菌素可能是一种有前景的抗酪氨酸酶药物和抗红色毛癣菌的新型活性化合物，红色毛癣菌可能是体癣的主要病原体。事实上，如果绿脓菌素次生代谢产物将用于实际药物治疗，它将满足对抗麻烦真菌感染的新型抗真菌天然药物的持续需求。
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