Froger-Gaillard B, Charrier A M, Thenet S, Ronot X, Adolphe M
Laboratoire de Pharmacologie Cellulaire, Paris, France.
Exp Cell Res. 1989 Aug;183(2):388-98. doi: 10.1016/0014-4827(89)90399-6.
Rabbit articular chondrocytes have a limited growth potential in vitro. After four passages in culture, chondrocytes have accomplished more than 50% of their life span. At this stage of culture, they are considered to be senescent-like, since a dramatic decrease in proliferative capacity and enhanced cell size and protein content are observed. These aged cells are, however, still able to respond to fibroblast growth factor (FGF). The addition of either acidic or basic FGF (10 ng/ml) to culture medium permitted an enhanced proliferation. The attenuation of FGF mitogenic activity during aging was not observed for both fractions. Moreover, when treated with acidic or basic FGF, aged chondrocytes had a smaller size and a lower protein content. The acidic FGF was less potent than the basic FGF in delaying the evolution of aged chondrocytes to senescence.
兔关节软骨细胞在体外的生长潜能有限。在培养传代四次后，软骨细胞已完成其寿命的50%以上。在这个培养阶段，它们被认为处于衰老样状态，因为观察到其增殖能力显著下降，细胞大小和蛋白质含量增加。然而，这些老化细胞仍能够对成纤维细胞生长因子（FGF）作出反应。向培养基中添加酸性或碱性FGF（10 ng/ml）可促进增殖。两种FGF组分在老化过程中均未观察到促有丝分裂活性的减弱。此外，当用酸性或碱性FGF处理时，老化软骨细胞的体积较小且蛋白质含量较低。在延缓老化软骨细胞向衰老演变方面，酸性FGF的效力低于碱性FGF。
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